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Lipidomics, 2009 Volume 1: Methods and Protocols Methods in Molecular Biology Series, Vol. 579

Langue : Anglais

Coordonnateur : Armstrong Donald

Couverture de l’ouvrage Lipidomics

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology? series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Part I: Shotgun and Global Lipidomics 1. Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics Michael A. Kiebish, Xianlin Han, and Thomas N. Seyfried 2. Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics Todd W. Mitchell 3. Global Analysis of Retina Lipids by Complementary Precursor Ion and Neutral Loss Mode Tandem Mass Spectrometry Julia V. Busik, Gavin E. Reid, and Todd A. Lydic 4. Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids Alfred N. Fonteh and Rachel D. Fisher Part II: Analytical Approaches 5. Lipid Profiling Using 2-Dimensional Heteronuclear Single Quantum Coherence NMR Engy A. Mahrous, Robin B. Lee, and Richard E. Lee 6. Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry Beate Fuchs, Ariane Nimptsch, Rosmarie Süß, and Jürgen Schiller 7. Lipidomics of the Red Cell in Diagnosis of Human Disorders Peter J. Quinn, Dominique Rainteau, and Claude Wolf 8. HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids Susanna L. Lundström, Fabio L. D'Alexandri, Kasem Nithipatikom, Jesper Z. Haeggström, Åsa M. Wheelock, and Craig E. Wheelock 9. Brain Phosphoinositide Extraction, Fractionation, and Analysis by MALDI-TOF MS Roy A. Johanson and Gerard T. Berry 10. Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry Giuseppe Astarita, Faizy Ahmed, and Daniele Piomelli 11. Lipidomic Analysis of Human Meibum Using HPLC-MSn Igor A. Butovich 12. Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry Timothy J. Garrett and William W.Dawson 13. A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface Wooki Kim, Robert S. Chapkin, Rola Barhoumi, and David W.L. Ma 14. Lipidomic Analysis of Prostanoids by Liquid Chromatography-Electrospray Tandem Mass Spectrometry Anna Nicolaou, Mojgan Masoodi, and Adnan Mir 15. Qualitative and Quantitative Analyses of Phospholipids by LC-MS for Lipidomics Hiroki Nakanishi, Hideo Ogiso, and Ryo Taguchi 16. Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting Zeev Wiesman and Bishnu P. Chapagain 17. Dynamics of Adipose Tissue Development by 2H2O Labeling Etienne Pouteau, Carine Beysen, Nabil Saad, and Scott Turner 18. Analysis of Lipid Particles from Yeast Melanie Connerth, Karlheinz Grillitsch, Harald Köfeler, and Günther Daum 19. Mammalian Fatty Acid Elongases Donald B. Jump 20. Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids : From Biomimetic Models to Biological Consequences Carla Ferreri and Chryssostomos Chatgilialoglu 21. On-Line Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids Michael C. Thomas, Todd W. Mitchell, and Stephen J. Blanksby 22. Comprehensive Quantitative Analysis of Bioactive Sphingolipids High-Performance Liquid Chromatography-Tandem Mass Spectrometry Jacek Bielawski, Jason S. Pierce, Justin Snider, Barbara Rembiesa, Zdzislaw M. Szulc, and Alicja Bielawska 23. The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids Marc Plante, Bruce Bailey, and Ian Acworth Part III: Lipid Maps 24. Non-Invasive Mapping of Lipids in Plant Tissue Using Magnetic Resonance

Regarding Volume 1 and 2:

Contains in-depth guides to isolation and processing of lipid samples, probes, lipid profiling, signaling, transport, and mapping

Provides examples utilizing key model systems

Presents oxidized lipids obtained from in vitro and in vivo biological material, redox platforms, high throughput systems, microscopy and imaging, and computer simulation

Serves as a comprehensive guide to technical applications

Explores bioinformatics strategy and statistical models for standardization of lipids

Includes supplementary material: sn.pub/extras

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Prix indicatif 174,06 €

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Date de parution :

Ouvrage de 550 p.

19.3x26 cm

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Prix indicatif 210,99 €

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