Lipidomics and Bioactive Lipids: Specialized Analytical Methods and Lipids in Disease

This volume in the well-established Methods in Enzymology series features methods for the study of lipids using mass spectrometry techniques. Articles in this volume cover topics such as Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosis; Measurement of eicosanoids in cancer tissues; Noninvasive Assessment of the Role of Cyclooxygenases in Cardiovascular HealthA Detailed HPLC/MS/MS Method; Lipidomics in Diabetes and the Metabolic Syndrome; LC-MS-MS Analysis of Neutral Eicosanoids; Quantification Of F2-Isoprostanes In Biological Fluids And Tissues As A Measure Of Oxidant Stress; Measurement of Products of Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans; Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography; Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS); Shotgun Lipidomics by Tandem Mass Spectrometry under Data-Dependent Acquisition Control; Identification of Intact Lipid Peroxides by Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS); Quantification of Cardiolipin by Liquid Chromatography Electrospray Ionization Mass Spectrometry.

Chapter 1
Liquid chromatography mass spectrometry for quantifying plasma lysophospholipids: potential biomarkers for cancer diagnosis
Mandi Murph, Tamotsu Tanaka, Jihai Pang, Edward Felix, Shuying Liu, Rosanne Trost, Andrew K. Godwin, Robert Newman and Gordon Mills

Chapter 2
Measurement of eicosanoids in cancer tissues
Dingzhi Wang and Raymond N. DuBois

Chapter 3
Noninvasive Assessment of the Role of Cyclooxygenases in Cardiovascular Health
A Detailed HPLC/MS/MS Method
Wen-Liang Song, John A. Lawson, Helen Zou, and Garret A. FitzGerald

Chapter 4
Lipidomics in Diabetes and the Metabolic Syndrome
Richard W. Gross, Xianlin Han

Chapter 5
LC-MS-MS Analysis of Neutral Eicosanoids
Philip J. Kingsley and Lawrence J. Marnett

Chapter 6
Quantification Of F2-Isoprostanes In Biological Fluids And Tissues As A Measure Of Oxidant Stress
Ginger L. Milne, Huiyong Yin, Joshua D. Brooks, Stephanie Sanchez, L. Jackson Roberts, II, and Jason D. Morrow

Chapter 7
Measurement of Products of Docosahexaenoic Acid Peroxidation, Neuroprostanes, and Neurofurans
Kyle O. Arneson and L. Jackson Roberts, II.

Chapter 8
Enantiomeric separation of hydroxy and hydroperoxy eicosanoids by chiral column chromatography
Claus Schneider, Zheyong Yu, William E. Boeglin, Yuxiang Zheng, and Alan R. Brash

Chapter 9
Targeted Chiral Lipidomics Analysis by Liquid Chromatography Electron Capture Atmospheric Pressure Chemical Ionization Mass Spectrometry (LC-ECAPCI/MS)
Seon Hwa Lee and Ian A. Blair

Chapter 10
Shotgun Lipidomics by Tandem Mass Spectrometry under Data-Dependent Acquisition Control
Dominik Schwudke, Gerhard Liebisch, Ronny Herzog, Gerd Schmitz and Andrej Shevchenko

Chapter 11
Identification of Intact Lipid Peroxides by Ag+ Coordination Ionspray Mass Spectrometry (CIS-MS)
Huiyong Yin and Ned A. Porter

Chapter 12
Quantification of Cardiolipin by Liquid Chromatography Electrospray Ionization Mass Spectrometry
Teresa A. Garrett*, Reza Kordestani, Christian R. H. Raetz

Graduate students, researchers in academics and industry studying biochemistry.