Lipases and Phospholipases, Softcover reprint of the original 1st ed. 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 861

Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes.

Part I: Overview of Lipases, Phospholipases and Related Enzymes

1. Lipases: An Overview                                                                  Leticia Casas Godoy, Sophie Duquesne, Florence Bordes, Georgina Sandoval and Alain Marty

2. Lipases or Esterases: Does it Really Matter? Toward a New Bio-Physico-Chemical Classification                                               Yassine Ben Ali, Robert Verger, and Abdelkarim Abousalham

3. Study of New Feruloyl Esterases to Understand Lipase Evolution: The Case of Bacillus flexus    Mónica Sánchez-González, Allan Blanco-Gámez, Roberto Parra-Saldívar, Juan Carlos Mateos-Díaz and Maria Isabel Estrada Alvarado

4. Phospholipases: An Overview                                                Ahmed Aloulou, Yassine Ben Ali, Sofiane Bezzine, Youssef Gargouri and Michael H. Gelb

Part II: Screening and Sources of Lipases, Phospholipases and Its Inhibitors

5. High-Throughput Sceening Method for Lipases/Esterases              Eduardo Mateos-Díaz, Jorge Alberto Rodríguez, María de los Ángeles Camacho-Ruiz, Juan Carlos Mateos-Díaz

6. Functional-Based Screening Methods for Lipases, Esterases and Phospholipases in Metagenomic Libraries

Dolores Reyes-Duarte, Manuel Ferrer, and Humberto García-Arellano

7. Plant Lipases:Partial Purification of C. papaya Lipase

Ivanna Rivera, Juan Carlos Mateos-Díaz, and Georgina Sandoval

 8. Plant Phospholipases: an Overview

Geliang Wang, Stephen Ryu, and Xuemin Wang

 9. Lipase and Phospholipase Inhibitors: Design and Applications

Mark E. Venable

10. High-Throughput Assay of Secreted Phospholipases A2 Inhibitors

Wallace Thompson, Rob C. Oslund, James Bollinger, Heather Ewing and Michael H. Gelb

Part III Cloning, Production, Purification and Characterization of Lipases and Phospholipases

11. Heterologous Expression Systems for Lipases: A Review

Francisco Valero

12. Streptomyces Phospholipase D: Cloning and Production

Yozo Nakazawa

13. Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses

Julian Gomez-Cambronero and Karen M. Henkels

 14. Lipases Production by Solid-State Fermentation: The Case of Rhizopus homothallicus in Perlite

Susana Velasco-Lozano, Tania Volke-Sepulveda, Ernesto Favela-Torres

 15. Lipases and Esterases from Extremophiles: Overview and Case Example of the Production and Purification of an Esterase from Thermus thermophilus HB27

Pablo Fuciños, Ramón González, Estrella Atanes, Ana Belem Fernández Sestelo, Nelson Pérez-Guerra, Lorenzo Pastrana and María Luisa Rúa

 16. Purification, Biochemical and Kinetic Properties of Recombinant
Staphylococcus aureus Lipase

Habib Horchani, Ahmed Fendri, Hanen Louatia, Adel Sayari, Youssef Gargouri and Robert Verger

 17. Phospholipase A2 Purification and Characterization; A Case Study

Aida Karray, Youssef Gargouri, Robert Verger and Sofiane Bezzine

 Part IV: Optimization of the Biocatalyst and Reaction Conditions

18. The Yeast Yarrowia lipolytica as a Generic Tool for Molecular Evolution of Lipases

Sophie Duquesne, Florence Bordes, Franck Fudalej, Jean-Marc Nicaud and Alain Martya

 19. Molecular Modeling of Lipase Binding to a Substrate-Water Interface

Christian C. Gruber and Jürgen Pleiss

 20. Different Strategies for Hyperactivation of Lipase Biocatalysts

Jose M. Palomo and Jose M. Guisan

 21. Rational Design of Immobilized Lipases and Phospholipases

Alberto del Monte Martínez and Bessy V. Cutiño Avila

 22. Thermodynamical Methods for the Optimization of Lipase Catalyzed Reactions

Edmundo Castillo, Alejandro Torres-Gavilán, Georgina Sandoval , Alain Marty

Part V: Applications of Lipases and Phopholipases

23. Lipases as Biocatalysts for the Synthesis of Structured Lipids  Ram Chandra Reddy Jala, Peng Hu, Tiankui Yang, Yuanrong Jiang, Yan Zheng, Xuebing Xu

24. Lipases-Catalyzed Modification of Phenolic Antioxidants          Pamela Torres, Dolores Reyes-Duarte, Antonio Ballesteros and Francisco J. Plou

25. Lipases as Catalysts in Synthesis of Fine Chemicals              Alicia Baldessari

26. Lipases in Green Chemistry: Acylation and Alcoholysis on Steroids and Nucleosides                                                                 Alicia Baldessari and Luis E. Iglesias

27. Lipases as Biocatalyst for Biodiesel Production                  Xiaohu Fan, Xochitl Niheus, Georgina Sandoval

28. Biopolymer Synthesis Catalyzed by Lipases                          Karla A. Barrera-Rivera, Arturo Flores-Carreón, Antonio Martínez-Richa

29. Phospholipases in Food Industry-A Review                          Víctor Casado, Diana Martín, Carlos Torres, Guillermo Reglero

30. Lipase and Phospholipase Biosensors: A Review                    Enrique J. Herrera-López

Details fundamental issues and new applications of lipases and phospholipases

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts