In Vivo Cellular Imaging Using Fluorescent Proteins, 2012 Methods and Protocols Methods in Molecular Biology Series, Vol. 872

The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.  What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocolsis the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.

Assessing Cancer Cell Migration And Metastatic Growth In Vivo In The Chick Embryo Using Fluorescence Intravital Imaging.- The Use Of Fluorescent Proteins For Intravital Imaging Of Tumor Cell Invasion.- High Resolution In Vivo Imaging Of Fluorescent Proteins Using Window Chamber Models.- In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models.- Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease.- Non-Invasive And Real-Time Fluorescence Imaging of Peritoneal Metastasis In Nude Mice.- Three-Dimensional  In Vivo Imaging Of Tumors Expressing  Red Fluorescent Proteins.- Real Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anti-Cancer Therapies.- In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus.- Real-Time Fluorescent Imaging of Abdominal, Pleural, and Lymphatic Metastases.- Real-Time Imaging Of Tumors Using Replication-Competent Light-Emitting Microorganisms.- GFP-Transgenic Animals For In Vivo Imaging: Rats, Rabbits And Pigs.- The Use Of Fluorescent Proteins For Developing Cancer-Specific Target Imaging Probes.- In Vivo Imaging Of The Developing Mouse Embryonic Vasculature.- Screening Reef Corals For Novel GFP-Type Fluorescent Proteins By Confocal Imaging.- What Does It Take To Improve Existing Fluorescent Proteins For In Vivo Imaging Applications?.- In Vivo Imaging of Oligonucleotide Delivery.- Subcellular Imaging In Vivo:  The Next GFP Revolution.