Imaging Gene Expression (2^nd Ed., 2nd ed. 2019) Methods and Protocols Methods in Molecular Biology Series, Vol. 2038

Imaging Single mRNA Molecules in Mammalian Cells using an Optimized MS2-MCP System.- Live-Cell Imaging of Long Non-Coding RNAs using Molecular Beacons.- Real-Time Observation of Localization and EXpression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene.- Visualization of Single mRNAs in Live Neurons.- Lightening Up Gene Activation in Living Drosophila Embryos.- Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis.- Imaging rRNA Methylation in Bacteria by MR-FISH.- Detection of mRNA Transfer between Mammalian Cells in Co-Culture by Single Molecule Fluorescent In Situ Hybridization (smFISH).- Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast.- Detection of mRNAs Anchored to the Nuclear Envelope during Export Inhibition in Living Cells.- Programmable Chromosome Painting with Oligopaints.- Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices.- Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.- Visualization of Endogenous Transcription Factors in Single Cells using an Antibody Electroporation-Based Imaging Approach.- Monitoring Transcription Factor Oligomerization in Single Living Cells by Numbers and Brightness Analysis.- Visualizing Transcription Factor Binding on Mitotic Chromosomes using Single Molecule Live-Cell Imaging.- Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells.