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Imaging and Spectroscopic Analysis of Living Cells Imaging Live Cells in Health and Disease

Langue : Anglais

Auteur :

This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike.

Part A: Viruses, Microbes and Parasites

Nanoscale imaging of microbial pathogens using atomic force microscopy

Challenges in imaging parasites

Part B: Disease States Real-time live imaging to study bacterial infections in vivo

Analysis of cellular membrane dynamics by following virus endocytosis

Imaging of live malaria blood stage parasites

Escherichia coli K1 invasion of human brain microvascular endothelial cells

Real-time imaging after cerebral ischemia

Imaging acute inflammation in zebrafish

Imaging protein oligomerization in neurodegeneration using BiEC

Imagng and quantifying the dyanmics of tumor-associated proteolysis

Part C: Other Techniques

Live cell imaging in vivo with fluorescent proteins

Visualization of gene expression

Imaging stem cell implant for cellular-based therapies

Live cell single molecule imaging to investigate early events in B lymphocyte signaling

A quantitative method for measuring phototoxicity of a live cell imaging microscope

High content screening of defined chemical libraries using normal and glioma-derived neural stem cell lines

High-content screening of primary neurons

Live-imaging fluorescent proteins in early mouse embryos

Comparing the methods for quantitative analysis of dendritic spine dynamics

Live-imaging of cell competition in drosophila imaginal epithelium

Live cell imaging of the oviduct

Computational estimation of leukocyte numbers in zebrafish

Four dimensional tracking of lymphocyte migration and interactions by two-photon microscopy

Biochemists, biophysicists, molecular biologists, analytical chemists, pharmacologists and physiologists
  • The third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells
  • The chapters provide hints and tricks not available in primary research publications
  • It is an invaluable resource for academics, researchers and students alike

Date de parution :

Ouvrage de 544 p.

15x22.8 cm

Disponible chez l'éditeur (délai d'approvisionnement : 14 jours).

171,65 €

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Thèmes d’Imaging and Spectroscopic Analysis of Living Cells :

Mots-clés :

3D Culture; 3D/4D Imaging; 4D microscopy; 4D time-lapse movies; Acute inflammation; and regeneration; Apoptosis; Atomic force microscopy; B cell signaling; bacterial virulence; Bimolecular fluorescence complementation assay; Biophotonic/bioluminescence imaging; Brain recovery; Cancer; Cell competition; cell migration; cell tracking; Cell trafficking; Cell-based tissue repair; cell-cell interaction; Chondrogenic differentiation; computer-based three-dimensional measurements; confocal microscopy; Cytoskeleton; Dendritic spine dynamics; Dendritic spine morphology; Drosophila; E; coli K1; Endocytosis; Firefly luciferase; FLIM; Fluorescence; Fluorescence microscopy; Fluorescence resonance energy transfer; Fluorescent protein; fluorescent proteins; four-dimensional imaging; FRET; Functional Imaging; GAP-43; germinal center; GFP; gliding motility; glioblastoma; HBMEC; High content screening; High-content screen; High-throughput microscopy; host-pathogen interactions; Hydrogen peroxide; HyPer; Imaging; immobilization; Infection; intact tissue; Intravital; Leukocyte; Live Cell Imaging; live cell microscopy; Live imaging; Live-cell imaging; live-imaging; lymphocyte; Macrophage; Malaria; MAME; Membrane traffic; Mesenchymal stem cell; Microscopy; Misfolding; Mitosis; Molecular imaging; Motor proteins; Mouse; Mouse embryo; Multiphoton; Nanomicrobiology; neural stem cells; Neurodegeneration; Neurodegenerative disorders; Neuroinflammation; Neutrophil; Oligomers; oocyte; Osteogenic differentiation; oviduct; P; falciparum; PDK1; Phototoxicity; PKB/Akt; Planar lipid bilayers; Plasmodium; Primary neuron; Primary neurons; Protease; Proteolysis; Rab5; Rab7; Receptor clustering; RFP; Single molecule tracking; Single particle tracking; sperm; sporozoite; Stroke; time lapse; TLR2; Total internal reflection microscopy; Transgenic mice; tubal transport; Tumor micro arrays; Tumor Microenvironment; Tumors; Two-color cells; two-photon microscopy; UPEC; Virus entry; Zebrafish