Human Monoclonal Antibodies, 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1060
The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.
1. Human Monoclonal Antibodies - The Residual Challenge of Antibody Immunogenicity
Herman Waldmann
2. Technical and Ethical Limitations in Making Human Monoclonal Antibodies
Mark C. Glassy and Rishab Gupta
3. Therapeutic Human Monoclonal Antibodies in Inflammatory Diseases
Sotirios Kotsovilis and Evangelos Andreakos
4. Therapeutic Human Monoclonal Antibodies Against Cancer
Jamie Jarboe, Anumeha Gupta, and Wasif Saif
5. Polyclonal and Monoclonal Antibodies in the Clinic
Bharath Wootla, Aleksandar Denic, and Moses Rodriguez
6. Production of Human Monoclonal Antibodies by the Epstein- Barr Virus Method
Michael Steinitz
7. Humanization and Simultaneous Optimization of Monoclonal Antibody
T. Kuramochi, T., Igawa, H. Tsunoda, and K. Hattori
8. Chimeric Antibodies
Kohei Kurosawa, Waka Lin, and Kunihiro Ohta
9. Recombinant Genetic Libraries and Human Monoclonal Antibodies
Jarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu
10. Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial Cytoplasm
Lilach Vaks and Itai Benhar
11. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG-Single Chain Fv Fusion
Dan Lu and Zhenping Zhu
12. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage Display
André Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, and Michael Hust
13. Antigen –Specific Human Monoclonal Antibodies from Transgenic Mice
Susana Magadán Mompó and África González-Fernández
14. Phage Display Technology for Human Monoclonal Antibodies
Cecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, and Daniele Sblattero
15. Antigen-Specific in vitro Immunization: A Source for Human Monoclonal Antibodies
Kosuke Tomimatsu and Sanetaka Shirahata
16. Methods for Radiolabelling of Monoclonal Antibodies
Vladimir Tolmachev, Anna Orlova, and Karl Andersson
17. Purification of Human Monoclonal Antibodies and Their Fragments
Thomas Muller-Späth and Massimo Morbidelli
18. Idiotype-Specific IVIG for Therapy of Autoimmune Diseases
Tomer Bashi, Miri Blank, and Yehuda Shoenfeld
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Ouvrage de 364 p.
17.8x25.4 cm
Ouvrage de 364 p.
17.8x25.4 cm