Gastrointestinal Physiology and Diseases, 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1422

This volume provides a comprehensive collection of  classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols booktargets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

CRISPR/Cas9 mediated Genome-editing of Mouse Small Intestinal Organoids.- Lentivirus-based Stable Gene Delivery into Intestinal Organoids.- Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cells.- An Air-Liquid Interface Culture System for 3D Organoid Culture of Diverse Primary Gastrointestinal Tissues.- Organotypical Tissue Cultures from Fetal and Neonatal Murine Colon.- Using Chamber Technique to Measure Intestinal Epithelial Permeability.- HPLC-based Metabolomic Analysis of Normal and Inflamed Gut.- NMR-based Metabolomic Analysis of Normal and Inflamed Gut.- Analysis of microRNA Levels in Intestinal Epithelial Cells.- Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the DrosophilaIntestine.- Imaging Inflammatory Hypoxia in the Murine Gut.- Label-free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography.- Near Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colon.- Visualization of Signaling Molecules during Neutrophil Recruitment in Transgenic Mice Expressing FRET Biosensors.- In Vivo Myeloperoxidase Imaging and Flow Cytometry Analysis of Intestinal Myeloid Cells.- Macrophage Isolation from the Mouse Small and Large Intestine.- Isolation and Functional Analysis of Lamina Propria Dendritic Cells from the Mouse Small Intestine.- Purification and Adoptive Transfer of Group 3 Gut Innate Lymphoid Cells.- Immunotherapy with iTreg and nTreg Cells in a Murine Model of Inflammatory Bowel Disease.- Isolation of Eosinophils from the Lamina Propria of the Murine Small Intestine.- Investigation of Host and Pathogen Contributions to Infectious Colitis Using the Citrobacter rodentium Mouse Model of Infection.- Murine Trinitrobenzoic Acid-induced Colitis as a Model of Crohn’s Disease.- Oxazolone-induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa.- The Mongolian Gerbil: A Robust Model of Helicobacter pylori-induced Gastric Inflammation and Cancer.- A Rapid Screenable Assay for Compounds that Protect Against Intestinal Injury in Zebrafish Larva.- AOM/DSS Model of Colitis-Associated Cancer.- Characterization of Colorectal Cancer Development in Apc ^min/+Mice.- Modeling Murine Gastric Metaplasia through Tamoxifen-Induced Acute Parietal Cell Loss.- The Hamster Buccal Pouch Model of Oral Carcinogenesis.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts