Flow Cytometry Protocols (3^rd Ed., Softcover reprint of the original 3rd ed. 2011) Methods in Molecular Biology Series, Vol. 699

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology? series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

Flow Cytometry: An Introduction.- Breaking the Dimensionality Barrier.- Quantitative Fluorescence Measurements with Multicolor Flow Cytometry.- Quantum Dots for Quantitative Flow Cytometry.- Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry.- Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization.- Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry.- Multiparameter Intracellular Cytokine Staining.- Phospho Flow Cytometry Methods for Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples.- Multiparametric Analysis of Apoptosis by Flow Cytometry.- Multiparameter Cell Cycle Analysis.- Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue.- Flow Cytometric-Based Identification of Immature Myeloerythroid Development.- Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia.- Flow Cytometry Assays in Primary Immunodeficiency Diseases.- Flow Cytometric Analysis of Microparticles.- Noncytotoxic DsRed Derivatives for Whole-Cell Labeling.- Flow Cytometric FRET Analysis of Protein Interaction.- Fluorescent Protein-Assisted Purification for Gene Expression Profiling.- Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type.- Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics.- Standard Practice for Cell Sorting in a BSL-3 Facility.- The Cytometric Future: It Ain’t Necessarily Flow!

Features polychromatic, quantitative, and high throughput flow cytometry protocols applicable to basic biological research as well as diagnostic medicine Paves the way for flow cytometry's use in the study of systems biology Includes extensive expert notes to ensure optimal results Includes supplementary material: sn.pub/extras