Essential Genes and Genomes, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2377

1. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9

            Barbara Mair, Michael Aregger, Amy H.Y. Tong, Katherine S.K. Chan, and Jason Moffat

2. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines

            Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, and Jason R. Cantor

3. Target Identification of Small Molecules Using Large-Scale CRISPR-Cas Mutagenesis Scanning of Essential Genes

            Bert Kwanten, Jasper Neggers, and Dirk Daelemans

4. Design and Generation of a CRISPR Interference System for Genetic Repression and Essential Gene Analysis in the Fungal Pathogen Candida albicans

            Lauren Wensing and Rebecca S. Shapiro

5. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens

            Luke DeHart, Oliver P. Yockey, and Jesse Bakke

6. A Simple Method that Combines CRISPR and AID to Quickly Generate Conditional Knockouts for Essential Genes in Various Vertebrate Cell Lines

            Kohei Nishimura and Tatsuo Fukagawa

7. New Method for Genome-Scale Functional Genomics Study in Bacteria with Superior Performance: CRISPR Interference Screen

            Xihao Liao, Xin-Hui Xing, and Chong Zhang

8. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria

            Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, and Cheng-Cai Zhang

9. A Method of Transposon Insertion Sequencing in Comprehensively Identifying Vibrio vulnificus Genes Required for Growth in Human Serum

            Miguel Carda-Diéguez and Carmen Amaro

10. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa

            Bradley E. Poulsen, Anne E. Clatworthy, and Deborah T. Hung

11. Analysis of Escherichia coli K1 Virulence Genes by Transposon-Directed Sequencing

            Alex J. McCarthy and Peter W. Taylor

12. Using Genome Scale Mutant Libraries to Identify Essential Genes

            Kevin S. Myers, Piyush Lal, Daniel R. Noguera, and Timothy J. Donohue

13. Identification and Analysis of Essential Genes in Streptococcus mutans with Transposon Sequencing

            Alejandro R. Walker and Robert C. Shields

14. The Use of TnSeq to Identify Essential Alphaproteobacterial Genes Reveals Operational Variability in Conserved Developmental and Cell Cycle Systems

            Gayatri Sharma and Patrick D. Curtis

15. A Proposed Framework to Identify Dispensable and Essential Functions in Bifidobacteria: Case Study of Bifidobacterium breve UCC2003 as a Prototype of Its Genus

            Lorena Ruiz, Francesca Bottacini, Lucie Semenec, Amy Cain, and Douwe van Sinderen

16. The Application of Transposon Insertion Sequencing in Identifying Essential Genes in B. fragilis 

            Yaligara Veeranagouda and Hannah M. Wexler

17. Repressible Promoter System to Study Essential Genes in Mycobacteria

            Francesca Boldrin and Riccardo Manganelli

18. A Single-Cell RNA-Seq-Based Approach for Genome-Wide Identification of Cell Essential Genes

            Yuqiu Lu and Shaolin Shi

19. Identification of Essential Genes in Caenorhabditis elegans with Lethal Mutations Maintained by Genetic Balancers

            Shicheng Yu, Chaoran Zheng, and Jeffrey Shih-Chieh Chu

20. A Screening Method to Identify Essential Yeast Genes for Responses Towards Spermine

            Mustapha Aouida and Dindial Ramotar

21. An Effective Pre-Processing Method for High-Quality Pan-Genome Analysis of Bacillus subtilis and Escherichia coli

            Hao Wu, Zhi-Kai Yang, Tong Yang, Dan Wang, Hao Luo, and Feng Gao

22. Analysis of Gene Essentiality from TnSeq Data Using Transit

            Thomas R. Ioerger

23. Geptop 2.0: Accurately Select Essential Genes from the List of Protein-Coding Genes in Prokaryotic Genomes

            Qing-Feng Wen, Wen Wei, and Feng-Biao Guo

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts