Drosophila Oogenesis, Softcover reprint of the original 1st ed. 2015 Methods and Protocols Methods in Molecular Biology Series, Vol. 1328

This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Drosophila melanogaster Oogenesis: An Overview

John M. McLaughlinand Diana P. Bratu

2. Basic Techniques in Drosophila Ovary Preparation

Letitia Thompson, Kristen Randolph, and Amanda Norvell

3. Mosaic Analysis in the DrosophilaMelanogaster Ovary

Thomas Rubin and Jean-René Huynh

4. Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary

Kaitlin M. Laws and Daniela Drummond-Barbosa

5. Culturing Drosophila Egg Chambers and Investigating Developmental Processes through Live Imaging

Lathiena Manning and Michelle Starz-Gaiano

6. Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

Mohit Prasad, Xiaobo Wang, Li He, Danfeng Cai, and Denise J. Montell

7. Visualizing Microtubule Networks during Drosophila Oogenesis using Fixed and Live Imaging

Kevin Legent, Nicolas Tissot, and Antoine Guichet

8. ­­Visualization of Actin Cytoskeletal Dynamics in Fixed and Live Drosophila Egg Chambers

Christopher M. Groen and Tina L. Tootle

9. Single Molecule RNA in situ Hybridization (smFISH) and Immunofluorescence (IF) in the Drosophila Egg Chamber

Livia V. Bayer,

10. Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries

Zehra F. Nizami, Ji-Long Liu, and Joseph G. Gall

11. Ultrastructural Analysis of Drosophila Ovaries by Electron Microscopy

Thomas R. Hurd, Carlos G. Sanchez, Felipe K. Teixeira, Chris Petzold, Kristen Dancel-Manning, Ju-Yu S. Wang, Ruth Lehmann, and Feng-Xia A. Liang

12.  Immuno-Electron Microscopy and Electron Microscopic in situ Hybridization for Visualizing piRNA Biogenesis Bodies in Drosophila Ovaries

Shinsuke Shibata, Yukiko Murota, Yoshinori Nishimoto, Mana Yoshimura, Toshihiro Nagai , Hideyuki Okano , and Mikiko C. Siomi

13. Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry

Ömür Y. Tastan and Ji-Long Liu

14. Detection of Cell Death and Phagocytosis in the Drosophila Ovary

Tracy L. Meehan, Alla Yalonetskaya, Tony F. Joudi, and Kimberly McCall

Dongyu Jia,Yi-Chun Huang, and Wu-Min Deng

16. Global Run-On Sequencing (GRO-seq) Library Preparation from Drosophila

Nikolay V. Rozhkov

17. Bioinformatics Analysis to Identify RNA-protein Interactions in Oogenesis

Ravinder Singh

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts