Clinical Applications of Mass Spectrometry in Drug Analysis, 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1383

This volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Chapters in the book cover a wide array of topics such as: quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma; quantitation of total Buprenorphine and Norbuprenorphine in Meconium; quantitation or Carisoprodol and Meprobamate in Urine; and quantitation of Tricyclic Antidepressants in Serum. Each chapter contains a brief introduction to the topic, clinical utility of the analyte(s), and useful notes to help laboratorians easily reproduce the protocols discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Clinical Applications of Mass Spectrometry in Drug Analysis: Methods and Protocols, is a great resource for laboratorians who are already using mass spectrometry or thinking of introducing this technology to their laboratories.

1. Mass Spectrometry in Clinical Laboratory: Applications in Therapeutic Drug Monitoring and Toxicology
Uttam Garg and Yan Victoria Zhang

2. Quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Matthew H. Slawson and Kamisha L. Johnson-Davis

3. Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS)
Jaime H. Noguez and James C. Ritchie

4. Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS
Dean C. Carlow, Heng Shi, and Ryan C. Schofield

5. Quantification of the Triazole Antifungal Compounds Voriconazole and Posaconazole in Human Serum or Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Alejandro R. Molinelli and Charles H. Rose, IV

6. Quantitation of Haloperidol, Fluphenazine, Perphenazine, and Thiothixene in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Matthew H. Slawson and Kamisha L. Johnson-Davis

7. Quantitation of Total Buprenorphine and Norbuprenorphine in Meconium by LC-MS/MS
Stephanie J. Marin and Gwendolyn A. McMillin
8. Quantitation of Buprenorphine, Norbuprenorphine, Buprenorphine Glucuronide, Norbuprenorphine Glucuronide, and Naloxone in Urine by LC-MS/MS
Stephanie J. Marin and Gwendolyn A. McMillin

9. A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantification of Plasma Busulfan
Shuang Deng, Michael Kiscoan, Clint Frazee, Susan Abdel-Rahman, Jignesh Dalal, and Uttam Garg

10. High-Throughput Quantitation of Busulfan in Plasma Using Ultra-Fast Solid Phase Extraction Tandem Mass Spectrometry (SPE-MS/MS)
Loralie J. Langman, Darlington Danso, Enger Robert, and Paul J. Jannetto

11. Quantification of 11-Carboxy-Delta-9-Tetrahydrocannbinol (THC-COOH) in Meconium Using Gas Chromatography Mass Spectrometry (GC/MS)
Judy Peat, Brehon Davis, Clint Frazee, and Uttam Garg

12. Quantitation of Carisoprodol and Meprobamate in Urine and Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Matthew H. Slawson and Kamisha L. Johnson-Davis

13. Cetirizine Quantification by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)
Ada Munar, Clint Frazee, Bridgette Jones, and Uttam Garg

14. Quantification of Docetaxel in Serum Using Turbulent Flow Liquid Chromatography Electrospray Tandem Mass Spectrometry (TFC-HPLC-ESI-MS/MS)
Christopher A. Crutchfield, Mark A. Marzinke and William Clarke
15. Comprehensive Urine Drug Screen by Gas-Chromatography Mass Spectrometry (GC/MS)
Bheemraj Ramoo, Melissa Funke, Clint Frazee, and Uttam Garg

16. Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometry
Judy Stone

17. High Resolution Mass Spectrometry for Untargeted Drug Screening
Alan H.B. Wu and Jennifer Colby

18. Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry(LC-MS/MS)
Matthew H. Slawson and Kamisha L. Johnson-Davis

19. Quantification of Hydroxychloroquine in Blood Using Turbulent Flow Liquid Chromatography-Tandem mass Soectrometry (TFLC-MS/MS)
Allison B. Chambliss, Anna K. Füzéry, and William A. Clarke

20. Quantification of Iohexol in Serum by High Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Faye B. Vicente, Gina Vespa, Alan Miller, and Shannon Haymond

21. Quantitation of Teriflunomide in Human Serum/Plasma Across a 40,000-Fold Concentration Range by LC/MS/MS
Geoffrey S. Rule, Alan L. Rockwood, and Kamisha L. Johnson-Davis

22. Determination of Menthol in Plasma and Urine by Gas Chromatography-Mass Spectrometry (GC/MS)
Judy Peat, Clint Frazee, Gregory Kearns, and Uttam Garg
23. Development of an Assay for Methotrexate and its Metabolites 7-Hydroxy Methotrexate and DAMPA in Serum by LC-MS/MS
Ryan C. Schofield, Lakshmi V. Ramanathan, Kazunori Murata, Martin Fleisher, Melissa S. Pessin, and Dean C. Carlow

24. Quantitative, Multidrug Pain Medication Testing by Liquid Chromatography – Tandem Mass Spectrometry (LC-MSMS)
Geza S. Bodor

25. Quantification of Free Phenytoin by Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS)
Judy Peat, Clint Frazee, and Uttam Garg

26. Detection of Stimulants and Narcotics by Liquid Chromatography Tandem Mass Spectrometry and Gas Chromatography Mass Spectrometry for Sports Doping Control
Brian D. Ahrens, Yulia Kucherova, and Anthony W. Butch

27. Quantification of Tricyclic Antidepressants in Serum Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)
Christopher A. Crutchfield, Autumn R. Breaud, and William Clarke 

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice form the experts