Chromatography and Isolation of Insect Hormones and Pheromones, 1990 The Chromatographic Society Symposium Series

The connection between the study of insects. their development. behaviour and biochemistry. and chromatography is perhaps not immediately obvious. However. this connection exists and it is of fundamental importance to our understanding of many areas of insect physiology. Insects range in size from small to minute and consequently the amounts of hormones or pheromones they produce are equally minute. Ultimately any attempt at understanding the processes which control development, social behaviour or the biochemistry of insects requires some means of isolating the tiny quantities of the hormones and pheromones responsible in sufficient quantity and purity for identification. The ability to devise novel techniques to separate these materials from frequently complex biological mixtures including precursors and metabolites and to devise detection systems for them is vital. Methods for the quantification of these substances at different stages in the life cycle. or in response to environmental change or stress. are then essential. Chromatography. both as a means for isolation and as a method for quantitative analysis. has "been an essential tool in these studies. This volume represents the outcome of a joint international symposium organized by the Chromatographic and Royal Entomological Societies at the University of Reading between the 21st and 23rd March 1989 aimed specifically at discussing the chromatography and isolation of insect hormones. pheromones and related substances.The papers presented at that meeting. and collected together here. covered many aspects of the subject including the chromatography of juvenile hormones. ecdysteroids. peptides. pheromones and semio­ chemicals.

Juvenile Hormones.- Endogenous Juvenile Hormone III Titres and In Vitro Rates of Hormone Biosynthesis by Corpora Allata During the Reproductive Cycle of Adult Female Periplaneta americana.- Use of Thin-Layer Chromatography, High Performance Liquid Chromatography and Gas Chromatography-Mass Spectrometry to Investigate the Relationship Between Juvenile Hormone Titre and Corpus Allatum Activity in Adult Male Periplaneta americana.- Use of Microderivatization Techniques in Combination with Thin-Layer Chromatography, Liquid Chromatography and Gas Chromatography-Mass Spectrometry for Investigation of Juvenile Hormones and Related Compounds.- The Precocene Antijuvenile Hormones (Allatotoxins): A Case History in Insect Toxicology.- The Regulation of the Corpora Allata by the Brain in the Adult Female American Cockroach, Periplaneta americana.- Ecdysteroids.- Chromatographic Separations of Ecdysone Acyl Esters and Their Application to the Distribution and Identification of Ecdysteroids in Adult House Crickets, Acheta domesticus.- Ecdysteroids in Eggs of Periplaneta americana: Rapid Analysis Using 3µ C18 Columns and the Binding of Ecdysteroids to Vitellin.- Advances in Ecdysteroid High Performance Liquid Chromatography.- Examination of Conditions in Supercritical Fluid Chromatography for Analysis of Ecdysteroids.- Current Status and Recent Advances in the Chromatography and Mass Spectrometry of Ecdysteroids.- Thin-Layer Chromatography of Ecdysteroids: Detection and Identification.- Overpressure Thin-Layer Chromatography of Ecdysteroids.- Selective Separation of 20,22-Dihydroxyecdysteroids from Insect and Plant Material with Immobilized Phenylboronic Acid.- Peptides.- Isolation and Characterization of a Diuretic Peptide, AP-I, from the Corpora Cardiaca of the House Cricket, Acheta domesticus.- Manduca Adipokinetic Hormone: Quantification Using High Performance Liquid Chromatography and Radioimmunoassay.- Extraction, Purification and Sequencing of Adipokinetic/Red Pigment-Concentrating Hormone-Family Peptides.- In Vitro Biosynthesis of Locust Adipokinetic Hormones: Isolation and Identification of the Bioactive Peptides and Their Prohormones.- A Strategy for the Isolation and Structural Characterization of Certain Insect Myotropic Peptides that Modify the Spontaneous Contractions of the Isolated Cockroach Hindgut.- Isolation and Identification of Neuropeptides in Locusta migratoria.- Adsorption Chromatography of Small C-Terminal Peptide Amides on Dihydroxyalkyl Bonded Silica High-Performance Liquid Chromatography Columns and Application to Purification of Insect Neuropeptides.- Isolation and Structural Characterization of Neuroparsins, Polytropic Neurohormones of the African Locust.- Isolation and Identification of a Sulfakinin-like Peptide, with Sequence Homology to Vertebrate Gastrin and Cholecystokinin, from the Brain of Locusta migratoria.- Characterization and Sequence Determination of Locust Brain cDNA Clones Selected by Antisera Raised Against Vertebrate Peptide Hormones.- A Comparative Immunocytochemical Study on the Presence of Cam-HrTH-II- and Lom-AKH-I-like Molecules in the Central Nervous System of Three Stick Insects Species: Carausius morosus, Sipyloidea sipylus and Extatosoma tiaratum.- Pheromones.- Insect Trail Pheromones: A Perspective of Progress.- Gas Chromatography Linked to Electroantennography: A Versatile Technique for Identifying Insect Semiochemicals..- Techniques for Isolation and Characterization of Volatile Semiochemicals of Phytophagous Insects.- The Use of Coupled Gas Chromatography: Electrophysiological Techniques in the Identification of Insect Pheromones.- Gas Chromatography-Mass Spectrometry in Insect Pheromone Identification: Three Extreme Case Histories.- High Performance Liquid Chromatography-Mass Spectrometry in the Analysis of Semiochemicals.- Analysis of Gland Secretions of Pentatomoidea (Heteroptera) by Gas Chromatography-Mass Spectrometry Techniques.- Kairomones of the Great European Spruce Bark Beetle Dendoroctonus micans.- Pygidial Glands of Primitive Australian Ants: A New Source of Odorous Chemicals.- Volatiles from Exocrine Glands of some Grassland Ants and Termites from Tropical West Africa.- Pattern Recognition.- A Computerized System for Pattern Recognition Designed for Gas Chromatograms.- Abstracts.- Compound Index.- Species Index.