Cellular and Subcellular Nanotechnology, 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 991

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

Nanoparticle-GFP “Chemical Nose” Sensor for Cancer Cells Identification.- A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor.- A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye.- Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains.- Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation.- Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments.- Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains.- A Method to Encapsulate Molecular Cargo within DNA Icosahedra.- Delivery of Plasmid DNA to Mammalian Cells using Polymer-Gold Nanorod Assemblies.- Lipophilic Formulated Gold Porphyrin Nanoparticles for Chemotherapy.- Mitochondria-specific Nano-Emulsified Therapy for Myocardial Protection against Doxorubicin-induced Cardiotoxicity.- Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane Events.- Characterization of Nanoparticle – Lipid Membrane Interactions using QCM-D.- Single-Cell Nanosurgery.- Single Quantum Dot Imaging in Living Cells.- Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging.- Monitoring the Degradation of Reduction Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry.- Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces.- Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery.- Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers.- Interactions of Nanoparticles with Proteins -Determination of Equilibrium Constants.- Tracing the Endocytic Pathways and Trafficking Kinetics of Cell Signaling Receptorsusing Single QD Nanoparticles.- Cellular Internalization of Quantum Dots.- Electrochemical Scanning Tunneling Microscopy and Spectroscopy for Single Molecule Investigation.- Intracellular Delivery of Biologically Active Proteins with Peptide-based Carriers.- Lipo-oligoarginine-based Intracellular Delivery.- Fluorescent Spherical Monodisperse Silica Core-shell Nanoparticles with a Protein-binding Biofunctional Shell.- Direct Quantification of PTD Transduction Using Real-Time Monitoring.- Genotoxic Assessment of Carbon Nanotubes.- Separation Science: Principles and Applications for the Analysis of Bionanoparticles by Asymmetrical Flow Field-Flow Fractionation (AF4).- Polymersomes-mediated Delivery of Fluorescent Probes for Targeted and Long Term Imaging in Live Cell Microscopy.- Protocol for the Preparation of Stimuli-responsive Gold Nanoparticles Capped with Elastin-based Pentapeptides.