Cell Imaging Techniques (2^nd Ed., Softcover reprint of the original 2nd ed. 2013) Methods and Protocols Methods in Molecular Biology Series, Vol. 931

Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters  covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls

 Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

Digital Images are Data – and Should be Treated as Such.- Epi-fluorescence Microscopy.-Live-Cell Migration and Adhesion Turnover Assays.-Multifluorescence Confocal Microscopy: Application for a Quantitative Analysis of HemostaticProteins in Human Venous Valve.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Colocalization Analysis in Fluorescence Microscopy.-A Time-Lapse Imaging Assay to Study Nuclear Envelope Breakdown.-Light Sheet Microscopy in Cell Biology.-Image-based High-throughput Screening for Inhibitors of Angiogenesis.-Intravital Microscopy to Image Membrane Trafficking in Live Rats.-Imaging Non-fluorescent Nanoparticles in Living Cells with Wavelength-Dependent.-Laser Scanning Cytometry. Principles and Applications: An Update.-Laser Capture Microdissection for Protein and NanoString RNA Analysis.-Viewing Dynamic Interactions of Proteins and a Model Lipid Membrane with Atomic Force Microscopy.-Mica Functionalization for Imaging of DNA and Protein-DNA Complexes with Atomic Force Microscopy.-Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation.-Atomic Force Microscopy Functional Imaging on Vascular Endothelial Cells.-Porosome: The Secretory NanoMachine in Cells.-Stereology andMorphometry of Lung Tissue.-A Novel Combined Imaging/Stereological Method for the Analysis of Human Sural Nerve Biopsies for Clinical Diagnosis.-Correlative Light–Electron Microscopy as a Tool to Study In vivo Dynamics and uUtrastructure of Intracellular Structures.-Photooxidation Technology for Correlative Light and Electron Microscopy.-Electron Microscopy of Endocytic Pathways.-Morphological Analysis of Autophagy.-Cytochemical Detection of Peroxisomes and Mitochondria.-Histochemical Detection of Lipid Droplets in Cultured Cells.-Environmental Scanning Electron Microscopy in Cell Biology.-Environmental Scanning Electron Microscopy Gold Immunolabeling in Cell Biology.-High Pressure Freezing for Scanning Transmission Electron Tomography Analysis of Cellular Organelles.-MALDI Imaging Mass Spectrometry for Direct Tissue Analysis.