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Cardiac Tissue Engineering, Softcover reprint of the original 1st ed. 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1181

Langue : Anglais

Coordonnateurs : Radisic Milica, Black III Lauren D.

Couverture de l’ouvrage Cardiac Tissue Engineering

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

1.       Second Generation Codon Optimized Minicircle (Comic) For Non-Viral Reprogramming Of Human Adult Fibroblasts

Sebastian Diecke, Leszek Lisowski, Nicholas M. Mordwinkin, Nigel G. Kooreman, and Joseph C. Wu


2.       Scalable Cardiac Differentiation Of Human Pluripotent Stem Cells as Microwell-Generated, Size Controlled Three-Dimensional Aggregates

Celine Bauwens and Mark Ungrin


3.       Preparation And Characterization Of Circulating Angiogenic Cells For Tissue Engineering Applications

Aleksandra Ostojic, Suzanne Crowe, Brian Mcneill, Marc Ruel, and Erik J. Suuronen


4.      Isolation And Expansion Of C-Kit Positive Cardiac Progenitor Cells By Magnetic Cell Sorting

Kristin M. French and Michael E. Davis


5.      Synthesis Of Aliphatic Polyester Hydrogel For Cardiac Tissue Engineering

Sanjiv Dhingra, Richard D. Weisel, and Ren-Ke Li


6.      Fabrication Of Pegylated Fibrinogen, A Versatile Injectable Hydrogel Biomaterial

Iris Mironi-Harpaz, Alexandra Berdichevski, and Dror Seliktar


7.       Native Cardiac Extracellular Matrix Hydrogels For Cultivation Of Human Stem Cell-Derived Cardiomyocytes

Donald O Freytes, John D O’Neill, Yi Duan-Arnold, Emily Wrona, and Gordana Vunjak-Novakovic


8.      Magnetically-Actuated Alginate Scaffold: A Novel Platform For Promoting Tissue Organization And Vascularisation

Yulia Sapir, Emil Ruvinov, Boris Polyak, and Smadar Cohen

9.      Shrink-Induced Biomimetic Wrinkled Substrates For Functional Cardiac Cell Alignment and Culture

Nicole Mendoza, Roger Tu, Aaron Chen, Eugene Lee, and Michelle Khine 


10.   Injectable ECM Scaffolds For Cardiac Repair

Todd Johnson, Rebecca Braden, and Karen Christman


11.   Generation Of Strip-Format Fibrin-Based Engineered Heart Tissue (EHT)

Sebastian Schaaf, Alexandra Eder, Ingra Vollert, Andrea Stöhr, Arne Hansen, and Thomas Eschenhagen


12.  Cell Tri-Culture For Cardiac Vascularization

Ayelet Lesman, Lior Gepstein, and Shulamit Levenberg


13.  Cell Sheet Technology For Cardiac Tissue Engineering

Yuji Haraguchi, Tatsuya Shimizu, Katsuhisa Matsuura, Hidekazu Sekine, Nobuyuki Tanaka, Kenjiro Tadakuma, Masayuki Yamato, Makoto Kaneko, and Teruo Okano


14.  Design And Fabrication Of Biological Wires

Jason W. Miklas, Sara S. Nunes, Boyang Zhang, and  Milica Radisic


15.   Collagen-Based Engineered Heart Muscle

Malte Tiburcy, Tim Meyer, Poh Loong Soong, and Wolfram-Hubertus Zimmermann


16.   Creation Of A Bioreactor For The Application Of Variable Amplitude Mechanical Stimulation Of Fibrin-Gel Based Engineered Cardiac Tissue

Kathy Y. Morgan and Lauren D. Black III


17.   Preparation Of Acellular Myocardial Scaffolds With Well-Preserved Cardiomyocyte Lacunae, And Method For Applying Mechanical And Electrical Simulation To Tissue Construct

Bo Wang, Lakiesha N. Williams,Amy L. De Jongh Curry, and Jun Liao


18.  Patch-Clamp Technique In ESC-Derived Cardiomyocytes

Jie Liu and Peter H. Backx


19.  Optogenetic Control Of Cardiomyocytes

Christina M. Ambrosi and Emilia Entcheva


20.  Methods For Assessing The Electromechanical Integration Of Human Pluripotent Stem Cell-Derived Cardiomyocyte Grafts

Wei-Zhong Zhu, Dominic Filice, Nathan J. Palpant, and Michael A. Laflamme


21.   Quantifying Electrical Interactions Between Cardiomyocytes and Other Cells In Micropatterned Cell Pairs

Hung Nguyen, Nima Badie, Luke Mcspadden, Dawn Pedrotty, and Nenad Bursac


Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 266 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 100,21 €

Ajouter au panier

Date de parution :

Ouvrage de 266 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

Prix indicatif 126,59 €

Ajouter au panier
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