Calcium-Binding Proteins and RAGE, 2013 From Structural Basics to Clinical Applications Methods in Molecular Biology Series, Vol. 963

Amajor direction in medical research leading to clinical applications  targets the regulation of intracellular calcium and the various human diseases associated with an altered homeostasis of this global second messenger. These diseases include, for example: cardiomyopathy, inflammation, brain disorders, diabetes and cancer. In Calcium-Binding Proteins and RAGE:from Structural Basics to ClinicalApplications,expert researchers in the field detail many of the methods which are now commonly used to study calcium binding proteins. These methods and techniques, such as calcium-measurements, screening methods, clinical chemistry, and therapy, are generally applicable to many other areas of basic and medical research as well as to diagnostics. Written in the highly successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,Calcium-Binding Proteins and RAGE:from Structural Basics to ClinicalApplications underlines the diagnostic and clinical importance of this family of proteins in human diseases and as drug targets.

Measurement of Intracellular Ca²⁺ Concentration in Single Cells using Ratiometric Calcium Dyes.-Divers Models of Divalent Cation Interaction to Calcium-binding Proteins: Techniques and Anthology.-Probing Ca²⁺ Binding Capability of Viral Proteins with the EF-hand Motif by Grafting Approach.-New Aspects of Calmodulin-calmodulin Binding Domains Recognition.-Purification and Characterization of the Human Cysteine-rich S100A3 Protein and its Pseudo Citrullinated Forms Expressed in Insect Cells.-X-ray Structural Analysis of S100 Proteins.-Purification and Stable Isotope Labeling of the Calcium and Integrin Binding Protein 1 for Structural and Functional NMR Studies.-Analysis of Calcium-induced Conformational Changes in Calcium-binding Allergens and Quantitative Determination of their IgE Binding Properties.-Longistatin, an EF-hand Ca²⁺-binding Protein From Vector Tick: Identification, Purification, and Characterization.-Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin.-NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides.-Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-hand Protein ALG-2 and Its Binding Partners.-Measuring binding of S100 proteins to RAGE by Surface Plasmon Resonance.-Phage Display Selection of Peptides that Target Calcium-Binding Proteins.-RAGE-mediated Cell Signalling.-RAGE Splicing Variants in Mammals.-Screening for Novel Calcium Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example.-In Vivo Screening of S100B Inhibitors for Melanoma Therapy.-Arrayed Primer Extension Microarray for the Analysis of Genes Associated with Congenital Stationary Night Blindness.-Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders.-Human Cell Line Authentication: The Critical First Step in any Project Using Human Cell Lines.-Detection of Ca²⁺-binding S100 Proteins in Human Saliva by HPLC-ESI-MS.-Clinical use of the Calcium-Binding S100B Protein.-Sensible use of High-sensitivity Troponin Assays.-S100A1 Gene Therapy in Small and Large Animals.