Caenorhabditis Elegans (2^nd Ed.) Molecular Genetics and Development

An updated edition of the classic Methods in Cell Biology volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Culturing methods and genetic proceduresGeneral methods of culture: Jim LewisMutagenesis: Phil AndersonGenetic screening methods: James Thomas, Eric Jorgensen, Eric LambieGenetic mapping methods: Ben Williams, Stephen Wicks (Boston College)Genetic mosaic analysis: Robert HermanUse of specialized chromosomes and rearrangements: Ann Rose, David BaillieGene knockouts: Shohei Mitani, Robert BarsteadRNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin

Genomics and BioinformaticsTranscriptional profiling: Stuart Kim, Don Moerman, Valerie ReinkeMicroRNA methods: Victor Ambros, Frank Slack, Amy PasquinelliAnalysis of RNA function: Marv Wickens, Judith KimbleAnalysis of the interactome: Marc VidalWormBase: Eric Schwartz, Paul Sternberg, Lincoln SteinStructural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama)Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, JTranscriptional regulatory networks: Marion Walhout

Markers for gene expression and protein localizationImmunofluorescence and antibodies: David MillerImaging: John White, William MohlerElectron Microscopy: David HallProteins and protein assemblies: Henry EpsteinAnalysis of morphogenesis: Jeff Hardin, Andrew ChisholmCell death: Monica Driscoll, Ding Xue

Researchers and students in cell, molecular and developmental biology

• Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
• Chapters written by leaders in the field
• Tried and true methods deliver busy researchers a one-stop compendium of essential protocols