Biophysical Methods in Cell Biology

This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Chapters cover such topics as AFM, traction force microscopy, digital holographic microscopy, single molecule imaging, video force microscopy and 3D multicolor super-resolution screening

1. Single-molecule Imaging of Cytoplasmic Dynein in vivo
Vaishnavi Ananthanarayanan and Iva M.Tolic
2. Single molecule imaging in live cell using gold nanoparticles
Cécile Leduc, Satyabrata Si, Jérémie J. Gautier, Zhenghong Gao, Edakkattuparambil S. Shibu,   Alexis Gautreau, Grégory Giannone, Laurent Cognet and Brahim Lounis
3. Quantitative Measurement of Transcription Dynamics in Living Cells
Adam M. Corrigan and Jonathan R. Chubb
4. An Easy-to-use Single-molecule Speckle Microscopy Enabling Nanometer-scale Flow and Wide-range Lifetime Measurement of Cellular Actin Filaments
Sawako Yamashiro, Hiroaki Mizuno, Naoki Watanabe
5. Dissecting Microtubule Structures by Laser Ablation
Franziska Decker and Jan Brugués
6. Quantifying Mitochondrial Content in Living Cells
Matheus Palhares Viana, Swee Lim, Susanne M. Rafelski
7. High-content 3D Multicolor Super-resolution Localization Microscopy
Pedro M Pereira, Pedro Almada, Ricardo Henriques
8. Superresolution Measurements in vivo: Imaging Drosophila Embryo by Photoactivated Localization Microscopy
Binh-An Truong Quang and Pierre-François Lenne
9. Refractive Index Measurements of Single, Spherical Cells Using Digital Holographic Microscopy
Mirjam Schürmann, Jana Scholze, Paul Müller, Chii J. Chan, Andrew E. Ekpenyong, Kevin J. Chalut, Jochen Guck
10. Construction, Imaging, and Analysis of FRET-Based Tension Sensors in Living Cells
Andrew S. LaCroix, Katheryn E. Rothenberg, Matthew E. Berginski, Aarti N. Urs, Brenton D. Hoffman
11. Single Cell Mechanics: the Parallel Plates Technique
Nathalie Bufi, Pauline Durand-Smet, Atef Asnacios
12. Atomic Force Microscopy-based Force Measurements on Animal Cells and Tissues
Hélène O. B. Gautier, Amelia J. Thompson, Sarra Achouri, David E. Koser, Kathrin Holtzmann, Emad Moeendarbary, and Kristian Franze
13. Measuring the Elasticity of Plant Cells with Atomic Force Microscopy
Siobhan A. Braybrook
14. Dual Pipette Aspiration: A Unique Tool For Studying Intercellular Adhesion
Maté Biro and Jean-Léon Maître
15. Measurement of Cell Traction Forces with ImageJ
Jean-Louis Martiel, Aldo Leal, Laetitia Kurzawa, Martial Balland, Irene Wang, Timothée Vignaud, Qingzong Tseng, Manuel Théry
16. Micropillar Substrates: a Tool for Studying Cell Mechanobiology
Mukund Gupta, Leyla Kocgozlu, Bibhu Ranjan Sarangi, Felix Margadant, Mohammed Ashraf and Benoit Ladoux
17. Mapping Forces and Kinematics During Collective Cell Migration
Xavier Serra-Picamal, Vito Conte, Raimon Sunyer, José J. Muñoz, and Xavier Trepat
18. Practical Aspects of the Cellular Force Inference Toolkit (CellFIT)
Jim H. Veldhuis, David Mashburn, M. Shane Hutson, and G. Wayne Brodland
19. Quantification of Collagen Contraction in Three-dimensional Cell Culture
Matthias Bussonnier, Sara Geraldo, Anthony Simon, Danijela Vignjevic, Timo Betz
20. Generation of Biocompatible Droplets for in vivo and in vitro Measurement of Cell-generated Mechanical Stresses
Otger Campas
21. Laser Induced Wounding of the Plasma Membrane and Methods to Study the Repair Process
Ana Joaquina Jimenez, Paolo Maiuri, Julie Lafaurie-Janvore, Franck Perez and Matthieu Piel
22. Electrofusion of Giant Unilamellar Vesicles to Cells
Dikla Raz-Ben Aroush, Shlomit Yehudai-Resheff and Kinneret Keren
23. Measurement and Manipulation of Cell Size Parameters in Fission Yeast
Yonatan Zegman, Daria Bonazzi and Nicolas Minc
24. Methods for Rectifying Cell Motions in vitro: Breaking Symmetry Using Microfabrication and Microfluidics
J. Comelles, V. Hortigüela, E. Martínez and D. Riveline
25. Analyzing Bacterial Movements on Surfaces
E. Laura Munteanu, Ingrid Spielman, Nicolas Biais
26. Advances in Single Cell Experimental Design Made Possible by Automated Imaging Platforms with Feedback Through Segmentation
Alex J. Crick, Eugenia Cammarota, Katie Moulang, Jurij Kotar and Pietro Cicuta