Biomedical Nanotechnology, Softcover reprint of the original 1st ed. 2011 Methods and Protocols Methods in Molecular Biology Series, Vol. 726

Due to their unique size-dependent properties, nanomaterials have the potential to revolutionize the detection, diagnosis, and treatment of disease by offering superior capabilities compared to conventionally-used materials.  Biomedical Nanotechnology: Methods and Protocols brings together experts from a wide variety of fields to provide a practical overview of biomedical nanotechnology, from the conception of novel materials in the laboratory to the application of such structures in the clinic. After a brief introductory chapter, the first section consists of protocol chapters which provide hands-on information on the synthesis of a variety of solution-phase and surface-bound nanomaterials and their application in sensing, imaging, and/or therapeutics, while the second section consists of a series of case studies and review chapters that discuss the toxicology of nanomaterials, the regulatory pathways to US Food and Drug Administration (FDA) approval of these materials, their patenting, marketing, and commercialization, and the legal and ethical issues surrounding their use.  Written in the highly successful Methods in Molecular Biology? series format, many chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and insightful tips on troubleshooting and avoiding known pitfalls.

Biomedical Nanotechnology.- Multiplexed Detection of Oligonucleotides with Biobarcoded Gold Nanoparticle Probes.- Molecular Detection of Biomarkers and Cells Using Magnetic Nanoparticles and Diagnostic Magnetic Resonance.- Real-Time Quantum Dot Tracking of Single Proteins.- Titanium Dioxide Nanoparticles in Advanced Imaging and Nanotherapeutics.- Surface Modification and Biomolecule Immobilization on Polymer Spheres for Biosensing Applications.- Multivalent Conjugation of Peptides, Proteins, and DNA to Semiconductor Quantum Dots.- A Single SnO₂ Nanowire-Based Microelectrode.- Biosensing Using Nanoelectromechanical Systems (NEMS).- Nano “Fly Paper” Technology for the Capture of Circulating Tumor Cells.- Polymeric Nanoparticles for Photodynamic Therapy.- Hydrogel Templates for the Fabrication of Homogeneous Polymer Microparticles.- Antibacterial Application of Engineered Bacteriophage Nanomedicines: Antibody-Targeted, Chloramphenicol Prodrug Loaded Bacteriophages for Inhibiting the Growth of Staphylococcus aureus Bacteria.- Viruses as Nanomaterials for Drug Delivery.- Applications of Carbon Nanotubes in Biomedical Studies.- Electrospun Nanofibrous Scaffolds for Engineering Soft Connective Tissues.- Peptide Amphiphiles and Porous Biodegradable Scaffolds for Tissue Regeneration in the Brain and Spinal Cord.- Computational Simulations of the Interaction of Lipid Membranes with DNA-Functionalized Gold Nanoparticles.- Cytotoxic Assessment of Carbon Nanotube Interaction with Cell Cultures.- Nanoparticle Toxicology:  Measurements of Pulmonary Hazard Effects Following Exposures to Nanoparticles.- Nanoparticle Therapeutics: FDA Approval, Clinical Trials, Regulatory Pathways, and Case Study.- Legislating the Laboratory?: Promotion and Precaution in a Nanomaterials Company.- Navigating the Patent Landscapes for Nanotechnology: English Gardens or Tangled Grounds?.- Scientific Entrepreneurship in the Materials and Life Science Industries.- Applying the Marketing Mix (5 P’s) to Bionanotechnology.- Managing the “Known Unknowns”: Theranostic Cancer Nanomedicine and Informed Consent.