Lavoisier S.A.S.
14 rue de Provigny
94236 Cachan cedex
FRANCE

Heures d'ouverture 08h30-12h30/13h30-17h30
Tél.: +33 (0)1 47 40 67 00
Fax: +33 (0)1 47 40 67 02


Url canonique : www.lavoisier.fr/livre/autre/atm-kinase/descriptif_3945991
Url courte ou permalien : www.lavoisier.fr/livre/notice.asp?ouvrage=3945991

0 article 

 Frais de port 0,01€*

Exclusivité web : à partir de 35 euros d’achat, frais de port à 1 centime pour les expéditions vers la France métropolitaine, la Suisse et l’UE avec Colissimo ou GLS

ATM Kinase, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1599

Langue : Anglais

Coordonnateur : Kozlov Sergei V.

Couverture de l’ouvrage ATM Kinase
Ataxia?telangiectasia (A-T) is a rare and severe genetic disorder affecting children. A-T is a multisystem disease characterized by progressive neurodegeneration, immunodeficiency and cancer predisposition. This detailed volume explores the ever expanding field of research into the ATM (ataxia-telangiectasia, mutated) gene and the role played by ATM kinase in DNA damage signaling and diverse cellular processes. What follows is a handy desktop reference for both seasoned A-T researchers and postgraduate students, as it demonstrates the breadth of recent developments in A-T studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Both classic and cutting-edge techniques are described, including ATM gene mutation detection, assays for radiosensitivity and radioresistant DNA synthesis, new methods to measure ATM kinase activity by imaging microscopy and high content screening as well as proteomics, phosphoproteomics and bioinformatics approaches to decipher ATM-dependent signalling pathways. Additional methods include generation of patient-specific stem cells and approaches to study ATM functions in the nervous system. 

Comprehensive and practical, ATM Kinase: Methods and Protocols aims to ignite and attract the interest of colleagues from diverse fields to A-T research in an effort to bring their expertise and fresh ideas to resolve many A-T puzzles still waiting to be pieced together and to alleviate the suffering of A-T children and their families.

1. Assaying Radiosensitivity of Ataxia-Telangiectasia

            Hailiang Hu, Shareef Nahas, and Richard A. Gatti

 

2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology

            Amanda W. Kijas and Martin F. Lavin

 

3. ATM Gene Mutation Detection Techniques and Functional Analysis

            Guillaume Rieunier, Catherine Dubois D’Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern

 

4. An HTRF® Assay for the Protein Kinase ATM

            Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater

 

5. ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform

            Catherine Bardelle and Joanna Boros

 

6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci

            Yi Chieh Lim

 

7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy

            Keiji Suzuki

 

8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging

            Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla

 

9. Zn(II)–Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein

            Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike

 

10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry

            Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov

 

11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)

            Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo

 

12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies

            Sun Yingli and Du Fengxia

 

13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays

            Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna

 

14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry

            Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz

 

15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling

            Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich

 

16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosphorylation

            Marina E. Borisova, Sebastian A. Wagner, and Petra Beli

 

17. Statistical Analysis of ATM-Dependent Signaling in Quantitative Mass Spectrometry Phosphoproteomics

            Ashley J. Waardenberg

 

18. ChIP Technique to Study Protein Dynamics at Defined DNA Double-Strand Breaks

            Jie Wen and Patrick Concannon

 

19. Studies of the DNA Damage Response by Using the Lac Operator/Repressor (LacO/LacR) Tethering System

            Rossana Piccinno, Marta Cipinska, and Vassilis Roukos

 

20. Imaging of Fluorescently-Tagged ATM Kinase at the Sites of DNA Double-Strand Breaks

            Anthony J. Davis, Shih-Ya Wang, David J. Chen, and Benjamin P.C. Chen

 

21. Live Cell Imaging to Study Real-Time ATM-Mediated Recruitment of DNA Repair Complexes to Sites of Ionizing Radiation-Induced DNA Damage

            Burkhard Jakob and Gisela Taucher Scholz

 

22. Analyzing Heterochromatic DNA Double Strand Break (DSB) Repair in Response to Ionizing Radiation

            Karolin Klement and Aaron A. Goodarzi

 

23. Phenotypic Analysis of ATM Protein Kinase in DNA Double-Strand Break Formation and Repair

            Elisabeth Mian and Lisa Wiesmüller

 

24. Monitoring DNA Repair Consequences of ATM Signaling Using Simultaneous Fluorescent Readouts

            Adrian Wiegmans

 

25. Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage

            Jurgen A. Marteijn, Wim Vermeulen, and Maria Tresini

 

26. Study of ATM Phosphorylation by Cdk5 in Neuronal Cells

            Hua She and Zixu Mao

 

27. DNA Damage Response in Human Stem Cells and Neural Descendants

            Jason M. Beckta, Bret R. Adams, and Kristoffer Valerie

 

28. A Patient-Specific Stem Cell Model to Investigate the Neurological Phenotype Observed in Ataxia-Telangiectasia

            Romal Stewart, Gautam Wali, Chris Perry, Martin Lavin, Francois Féron, Alan Mackay-Sim, and Ratneswary Sutharsan

 

29. Lentiviral Reprogramming of A-T Patient Fibroblasts to Induced Pluripotent Stem Cells

            Sam Nayler, Sergei Kozlov, Martin Lavin, and Ernst Wolvetang

 

30. Monitoring the ATM-Mediated DNA Damage Response in the Cerebellum Using Organotypic Cultures

            Efrat Tal and Yosef Shiloh

Includes cutting-edge methods and protocols for the study of ataxia-telangiectasia research

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

Ouvrage de 437 p.

17.8x25.4 cm

Sous réserve de disponibilité chez l'éditeur.

158,24 €

Ajouter au panier