Array Comparative Genomic Hybridization, Softcover reprint of the original 1st ed. 2013 Protocols and Applications Methods in Molecular Biology Series, Vol. 973
The earliest descriptions of human chromosomes initiated the genomics revolution that is now upon us. Array Comparative Genomic Hybridization: Protocols and Applicationsexplores the scope of what is now possible as far as documenting abnormalities associated with several human cancers. While the technology for interrogating the human genome continues to evolve, refinement of comparative genomic hybridization (CGH) using array CGH and related technologies have provided enormous insight into human cancers at an affordable scale. Written in the successful Methods in Molecular Biology? series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Array Comparative Genomic Hybridization: Protocols and Applicationsprovides researchers with well-honed methodologies to learn these techniques for their own use in research or clinical diagnostic laboratories.
1. Array Comparative Genomic Hybridization: An Overview of Protocols, Applications and Technology Trends
Diponkar Banerjee
2. Ultra Dense Array CGH and Discovery of Micro-Copy Number Alterations and Gene Fusions in the Cancer Genome
Ewa Przybytkowski, Adriana Aguilar-Mahecha, Sheida Nabavi, Peter J. Tonellato, and Mark Basik
3. Epigenomics: Sequencing the Methylome
Martin Hirst
4. Application of Array Comparative Genomic Hybridization in Chronic Myeloid Leukemia
Seonyang Park, Youngil Koh, Seung-Hyun Jung, and Yeun-Jun Chung
5. The Use of Cytogenetic Microarrays in Myelodysplastic Syndrome Characterization
Lisa G. Shaffer, Blake C. Ballif, and Roger A. Schultz
6. CGH Protocols - Chronic Lymphocytic Leukemia
Esteban Braggio, Rafael Fonseca, and Neil E. Kay
7. Analysis of Acquire Genomic Copy Number Aberrations and Regions of Loss of Heterozygosity in Acute Myelogenous Leukemia Fenomes using Affymetrix SNP 6.0 Arrays and Supporting Software Tools
Peter D. Ouillete, Kerby A. Shedden, Cheng Li, and Sami N. Malek
8. Clinical Applications of BAC Array-CGH to the Study of Diffuse Large B-Cell Lymphomas
Cristina Robledo, Juan Luis García, and Jesús M. Hernández
9. Genomic Profiling of Mantle Cell Lymphoma
Melissa Rieger and Jose A. Martinez-Climent
10. Copy Number Analysis in EBV-Positive Nodal Peripheral T-Cell Lymphoma, Unspecified
Kyusam Choi, Hyunjeong Ju, and Young Hyeh Ko
11. Mycosis Fungoides and Sézary Syndrome
Blanca Espinet and Rocío Salgado
12. Array CGH Reveals Clonal Evolution of Adult T Cell Leukemia/Lymphoma
Akira Umino and Masao Seto
13. Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma
K. Szuhai, R. Van Doorn, and C.P. Tensen
14. Genome Wide DNA-Profiling of HIV-Related B-Cell Lymphomas
Andrea Rinaldi, Daniela Capello, Emanuele Zucca, Gianluca Gaidano, and Franceso Bertoni
15. Array Comparative Genomic Hybridization in Osteosarcoma
Bekim Sadijovi, Paul C. Park, Shamini Selvarajah, and Maria Zielenska
16. Comparative Genomic Hybridization of Wilms' Tumor
Shahrad Rod Rassekh and Evica Rajcan-Separovic
17. Array-CGH in Childhood MDS
Marcel Tauscher, Inka Praulich, and Doris Steinemann
18. Non-Familial Breast Cancer Subtypes
Markus Ringnér, Johan Staaf, and Göran Jönsson
19. Applications of Array-CGH for Lung Cancer
Kenneth J. Craddock, Wan L. Lam, and Ming-Sound Tsao
20. Array CGH in Brain Tumours
Gayatry Mohapatra, Julia Sharma, and Stephen Yip
21. Finding Common Regions of Alteration in Copy Number Data
Oscar M. Rueda, Ramon Diaz-Uriarte, and Carlos Caldas
22. Distinguishing Somatic and Germline Copy Number Events in Cancer Patient DNA Hybridized to Whole Genome SNP Genotyping Arrays
Gavin Ha and Sohrab ShahIncludes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Ouvrage de 382 p.
17.8x25.4 cm
Ouvrage de 382 p.
17.8x25.4 cm
Mots-clés :
CGH protocols; array CGH; cancer genome; epigenomics; human neoplasia