Applications of spectroscopy to biomedical problems, (Faraday discussions, N° 126)

The volume brings together biochemists and biomedical scientists who use spectroscopy to investigate detailed properties of systems, ranging from single proteins to cells, with groups applying spectroscopy to medical diagnosis. The emphasis is on the assignment and fundamental understanding of spectra leading to a better informed application. Work is presented in the following areas: Infrared and Raman spectroscopy, Terahertz spectroscopy, Spectroscopic imaging, Fluorescence techniques, Circular dichroism, NMR spectroscopic imaging, EPR spectroscopy, Mössbauer spectroscopy. Faraday Discussions documents a long-established series of Faraday Discussion meetings which provide a unique international forum for the exchange of views and newly acquired results in developing areas of physical chemistry, biophysical chemistry and chemical physics. The papers presented are published in the Faraday Discussion volume together with a record of the discussion contributions made at the meeting. Faraday Discussions therefore provide an important record of current international knowledge and views in the field concerned.

From biomolecules to biodiagnostics: Spectroscopy does it all, Histological and microscopy FT-IR imaging study on the proliferative activity and angiogenesis in head and neck tumours. Infrared microscopy of epithelial cancer cells in whole tissues and in tissue culture, using synchrotron radiation. The combined application of FTIR microspectroscopy and ToF-SIMS imaging in the study of prostate cancer. Structural organisation of nucleic acids from tumour cells. Discrimination between neurochemical and macromolecular signals in human frontal lobes using short echo time proton magnetic resonance spectroscopy. In vivo EPR spectroscopy: biomedical and potential diagnostic applications. Mössbauer spectroscopy in biomedical research. Raman spectroscopy for identification of epithelial cancers. Bone tissue ultrastructural response to elastic deformation probed by Raman spectroscopy. Caveolin-1 isoform reorganization studied by image correlation spectroscopy. Application of fluorescence correlation spectroscopy to the measurement of agonist binding to a G-protein coupled receptor at the single cell level. g-Factor analysis of protein secondary structure in solutions and thin films. Applications of extended ultra-violet circular dichroism spectroscopy in biology and medicine. Biomedical applications of synchrotron radiation circular dichroism spectroscopy: Identification of mutant proteins associated with disease and development of a reference database for fold motifs. Terahertz pulsed imaging and spectroscopy for biomedical and pharmaceutical applications. Spectroscopic diagnosis and imaging of invisible pre-cancer. Multiple labelled nanoparticles for bio detection. Imaging capabilities of synchrotron infrared microspectroscopy.