Advances in Tracer Methodology, 1965 Volume 2 A collection of papers presented at the Sixth, Seventh, and Eight Symposia on Tracer Methodology plus other papers selected by the editor

Advances in Tracer Methodology, Volume 2, records the pro­ ceedings of the 6th, 7th, and 8th Symposia on Advances in Tracer Methodology. These Symposia, which are part of a continuing series sponsored by the New England Nuclear Corp. and the Packard Instrument Company, Inc., are devoted to the entire isotope tracer field: preparation and analysis of labeled compounds, applications in the chemical, biochemical, and clinical fields, and health physics considerations associated with tracer work. The papers in this volume reflect certain trends which can be noted in the tracer field: increasing reliance on biochemical methods for labeling esoteric compounds, growing awareness of the problems of radiation decomposition, the use of advanced instrumentation for the analysis and detection of radioactive substrates, and the rapidly growing application of tracer compounds to clinical diagnosis. The three Symposia at which the papers were presented were held on the following dates: DATE LOCATION CHAIRMAN 11/16/62 New York City Daniel Steinberg, Chief Laboratory of Ce1lular PhYSiology and Metabolism, National Heart Institute, Bethesda, Maryland 3/8/63 Los Angeles Martin Kamen University of California San Diego, California 11/8/63 Chicago Joseph L. Rabinowitz Veterans Administration Hospital Philadelphia, Pennsylvania Once again, it is the editor's pleasure to acknowledge the help of his colleagues in planning and running the various Symposia, as well as the excellent cooperation of the chairmen, who acquitted themselves in their appointed tasks in a most efficient manner, With humor and understanding. S. R. Boston, Mass.

Early History of Carbon-14.- Methods for Labeling Compounds.- Conditions for High Yield in the Labeling of Hydrocarbons by Exchange.- C14-Labeling Using Carbene Insertion—Application to Saturated Hydrocarbons.- Biosynthesis of Labeled Carbohydrates and Other Compounds of Biochemical Interest.- The Preparation of Labeled Albumins for Turnover Studies.- The Biosynthesis of C14- and H3-Labeled Insulin.- Purification of High Specific Activity Acetic — H3 Anhydride.- Special Analytical Techniques.- Automatic Counting of Radioactivity on Two-Dimensional Paper Chromatograms.- A Convenient Method for the Determination of Metabolically Liberated C14O2.- Liquid Scintillation Counting of C14-Labeled Amino Acids on Paper, Using Trinitrobenzene- 1-Sulfonic Acid, and an Improved Combustion Apparatus.- Zonal Scanning of Thin-Layer Chromatograms.- Continuous Scintillation Counting of Amino Acid Analyzer Column Eluates.- Some Techniques of Radioactive Gas Chromatography for Lipid Research.- The Microchemical Identification of Steroids from Biological Media.- Isotope Fractionation in the Absorption Chromatography of Doubly-Labeled Compounds.- Quench Correction by Automatic External Standardization.- Biochemical Applications.- Analytical Studies Using Low Levels of Carbon-14 and Tritium: A Method for Determining the Labeling Pattern in myo-Inositol.- Multiple Labeling to Determine Metabolic Pathways: Use of Labeling Ratios.- Diffusion of Radioactively Labeled Molecules in Heart Muscle.- Use of Labeled Nonmetabolized Amino Acids in Biochemical Research.- The Use of DMO-2-C14 (5,5,- Dimethyl- 2,4-Oxazolidinedione-2-C14) and Inulin-Carboxyl-C14 for Measurement of Intracellular pH.- Clinical Applications.- AEC Licensing of Radioisotopes for Clinical Research.- The Preparation ofRadioactive Chemicals for Clinical Use.- Clinical Applications of Aldosterone In Vivo Secretion Rate.- Steroid Secretory Mixtures in Man.- Isotopic Methods for Steroids in Human Blood.- Estimation of Alternative Metabolic Pathways of Catecholamines in Man.- Use of Tracers in the Study of Biogenic Amine Compartments.- Evaluation of Rates of Secretion and of Interconversion of Steroid Hormones.- Diisopropylfluorophosphate-P32 (DFP-P32) as a Label for Granulocytes.- Clinical Use of Liquid-Type Whole-Body Radioactivity Detectors.- Paired Labeling with I125 and I131.- The In Vivo Use of Doubly-Labeled Glucose to Determine Cerebral Metabolism in Humans.- Carbon-14 Fat Oxidation Test: A New Method for Measuring Fat Utilization in the Human.- Health Physics.- Coordinated Design of Radioisotope Laboratories.