Advanced techniques in diagnostic cellular pathology

There is an increasing need for an understanding of the scientific basis of cellular pathology to facilitate a growing trend towards inter-disciplinary understanding in biomedical sciences. Advanced Techniques in Diagnostic Cellular Pathology provides biomedical science students and professionals with the necessary background information, principles and methodology of advanced techniques to assist diagnosis in the modern cellular pathology laboratory. Each chapter is carefully structured to introduce the very latest techniques and describe their clinical purpose, principle, method and application in cellular pathology. The advantages of various methods for preparing, observing and demonstrating cells and tissues employed to assist in diagnosis are explored, in addition to the use of quantitative methods in the detection and diagnosis of disease. An accompanying web site presents full colour digital images of microscope slides, advanced protocols that the book can reference, appendices and web links. This book is invaluable for newly qualified postgraduates needing to get up to speed on a broad range of analytical techniques. It is also of interest to professionals working in diagnostic, industrial and research laboratories for continual professional development and training.

Supplementary Materials. 1 Virtual Microscopy. Digital (virtual) microscopy: equipment for implementation. The virtual slide format. Image serving and viewing. Applications of virtual microscopy. Virtual microscopy in education. Computational aspects of virtual microscopy. 2 Cytopathology. Mary Hannon-Fletcher. Basic principles. Cytodiagnosis. Gynaecological cytopathology. 3 Flow Cytometry. Sample preparation. Principles of the flow cytometer. Clinical applications. The future for flow cytometry. 4 Immunocytochemistry. Basic principles. Clinical immunocytochemistry. Microarray-based Comparative Genomic Hybridization. Principles of array CGH. aCGH platforms. Choosing the right platform. Analysis and validation. Finding the target. Data integration. Clinical applications. 6 Tissue In SituHybridization. ISH probes. Probe labels. ISH detection systems. Tissue preparation and the ISH procedure. Signal amplification. ISH controls. Clinical applications of tissue ISH. ISH automation. Image capture and analysis. Recent developments and future directions in tissue ISH. Conclusions. Index.