Advanced diagnostic methods in pathology

This book provides for the practicing pathologist and the researcher the information which is necessary to make decisions regarding the incorporation of immunohistochemical, cytometric and molecular biologic tests into clinical practice. In the early chapters, the book introduces basic biological principals that are useful in putting these methods into context, such as the functions of oncogenes and tumour suppressor genes and the structures of antigens used in diagnostic pathology. In the next few chapters, the basic methods of immunocytochemistry, in-situ hybridization, Southern blotting, PCR and other methods are presented, together with well-validated protocols that may be used in the clinical laboratory. In the third part of the book, an organ-system based exposition of common diagnostic issues is presented, which incorporates cytogenetic, molecular biological, immunohistochemical and cytometric information. In addition, selected protocols are provided.

PART. PRINCIPLES. Oncogenes and Tumor Suppressor Genes. Antigens PART. PROTOCOLS. Immunohistochemistry and In Situ Hybridization Techniques. Solution Hybridization and Blotting Techniques. Amplification Methods. Flow Cytometry for Antigen Expression PART. PRACTICE. Infectious Diseases. Cardiovascular System. Lung, Mediastinum, and Pleura. Kidney. Testes, Bladder, Prostate. Digestive System. Breast and Female Reproductive Tract. Endocrine System. Hematologic and Lymphoid System. Bones and Soft Tissue. Skin. Cytopathology APPENDICES

Pathologists, particularly those interested in molecular pathology