Advances in Food Biotechnology

The application of biotechnology in the food sciences has led to an increase in food production, and enhanced the quality and safety of food. Food biotechnology is a dynamic field, and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food.

Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics:

• GMOs and food security issues
• Applications of enzymes in food processing
• Fermentation technology
• Functional food and nutraceuticals
• Valorization of food waste
• Detection and control of foodborne pathogens
• Emerging techniques in food processing

Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research, regulatory agencies and those who are studying and teaching food biotechnology.

Also available from Wiley
Nanotechnology and Functional Foods: Effective Delivery of Bioactive Ingredients
Edited by Cristina M. Sabliov, Hongda Chen, Rickey Y. Yada
ISBN: 978-1-118-46220-1

Fundamentals of Food Biotechnology, 2nd Edition
Byong H. Lee
ISBN: 978-1-118-38495-4

Preface xxvii

I GLOBAL FOOD SECURITY: ARE GMOS THE SOLUTION TO THE FOOD SECURITY ISSUE? 1

1 Biotechnological Approaches for Nutritionally Enhanced Food Crop Production 3
Kathleen L. Hefferon and Abdullah Makhzoum

1.1 Introduction 3

1.2 The Case for Biofortified Food 3

1.3 Nutritionally Enhanced Feed Crops 6

1.4 Plants with Other Health Benefits 6

1.5 Biopharmaceuticals Produced in Plants 6

1.6 Genome Editing for Nutritionally Enhanced Plants 7

1.7 Epigenetics and Nutritionally Enhanced Plants 7

1.8 Risk Assessment and Regulation of Nutritionally Enhanced Crops 9

1.9 Conclusions 9

References 10

2 Current and Emerging Applications of Metabolomics in the Field of Agricultural Biotechnology 13
Camilla B. Hill, Daniel A. Dias, and Ute Roessner

2.1 Introduction 13

2.2 Metabolomics of Cereals for Food Production 16

2.3 Metabolomics and its Application in the Production of Wine 18

2.4 Final Remarks 23

Acknowledgements 23

References 23

3 Safety Assessment of Genetically Modified Foods 27
Gijs A. Kleter and Maryvon Y. Noordam

3.1 Introduction 27

3.2 Safety Assessment of GM-Crop-Derived Foods 28

3.3 Recurrent Items Addressed during the Food and Feed Safety Assessment 28

3.4 Outlook and Future Challenges 35

3.5 Conclusions 36

Acknowledgements 36

References 36

4 Towards a Universal Molecular Approach for the Quality Control of New Foodstuffs 37
Andrea Galimberti, Anna Sandionigi, Antonia Bruno, Ilaria Bruni, Michela Barbuto, Maurizio Casiraghi, and Massimo Labra

4.1 Food Quality and Safety Assessment in the Era of Genomics 37

4.2 DNA Barcoding: General Characteristics and Applications for the Analysis of Modern Foodstuffs 38

4.3 Microbiological Composition of Foodstuffs 38

4.4 Pathogenic Microorganisms and Food Spoilage 43

4.5 Towards a Molecular Identification of Food-Related Microorganisms 44

4.6 Towards a Standardized Molecular Identification of Food Raw Materials 45

4.7 Next-Generation Technologies to Characterize Complex Food Matrices and their Microbiome 50

4.8 Conclusions 51

References 51

5 Mass Spectrometry-Based Approaches in Food Safety 61
Pasquale Ferranti and Gianluca Picariello

5.1 Background 61

5.2 Instrumentation 61

5.3 Mass Spectrometry and Food Safety 63

5.4 Effects of Technological Processing 64

5.5 Microbiological Issues 65

5.6 Genetically Modified Organisms 65

5.7 Food Allergy 66

5.8 Food Metabolomics 67

5.9 Food Lipidomics 67

5.10 Current Challenges and Perspectives 68

References 68

6 Feeding the World: Are Biotechnologies the Solution? 71
Yves Bertheau

6.1 Introduction 71

6.2 Current Situation 72

6.3 Proposed Solutions 76

6.4 Conclusion 94

References 95

II APPLICATION OF ENZYMES IN THE FOOD INDUSTRY 103

7 Application of Microbial Enzymes in the Food Industry 105
Alane Beatriz Vermelho, Verônica Cardoso, Rodrigo Pires Nascimento, Anderson S. Pinheiro, and Igor Rodrigues de Almeida

7.1 Introduction 105

7.2 The Main Enzymes 106

7.3 Main Microorganism Producers of Enzymes 111

7.4 Marine Microbial Enzymes 115

7.5 Dairy Industry 116

7.6 Microbial Enzymes Applied in the Beverage Industry 118

7.7 Animal Feed 121

7.8 Targeting Microbial Enzymes of Industrial Interest 123

7.9 Mathematical Models for Enhanced Enzyme Production 124

Acknowledgements 124

References 125

8 Enzymatic Modification of Proteins and Starches for Gluten-Free and Low-Glycaemic-Index Foods for Special Dietary Uses 133
A.M. Calderón de la Barca, A.R. Islas-Rubio, N.G. Heredia, and F. Cabrera-Chávez

8.1 Introduction 133

8.2 Foods for Special Dietary Uses 134

8.3 Wheat Constituents that may Trigger Adverse Reactions 134

8.4 Gluten Proteins: Role in Pathogenesis of Gluten-Related Disorders 135

8.5 Enzymatic Modification of Proteins 136

8.6 Polysaccharides and the Glucose Response 139

8.7 Polysaccharide Enzymatic Modification 140

8.8 Conclusions 141

References 141

9 Enzyme Immobilization and its Application in the Food Industry 145
Ahmad Homaei

9.1 Introduction 145

9.2 History of Enzyme Immobilization 145

9.3 Carrier Materials for Enzyme Immobilization 146

9.4 Enzyme Immobilization Techniques 148

9.5 Commercialization and Use of Immobilized Enzymes in the Food Industry 153

9.6 Conclusions 159

References 159

10 Enzymes for Food and Beverage Industries: Current Situation, Challenges and Perspectives 165
Antonella Amore and Vincenza Faraco

10.1 Introduction 165

10.2 Application of Enzymes in Food and Beverage Industries 166

10.3 Tools to Enhance Use of Food Enzymes 178

10.4 Conclusions, Challenges and Perspectives 182

References 183

11 Enzymes Inhibitors: Food and Non-Food Impacts 191
Nana Akyaa Ackaah-Gyasi, Yi Zhang, and Benjamin K. Simpson

11.1 Introduction 191

11.2 Types of Enzyme Inhibitors 191

11.3 Sources of Enzyme Inhibitors 194

11.4 Isolation and Purification of some Naturally Occurring Enzyme Inhibitors 196

11.5 Mechanisms of Action 196

11.6 Food Uses of Enzyme Inhibitors 198

11.7 Health and Biomedical Uses of Inhibitors 200

11.8 Future of Enzyme Inhibitors 201

References 202

12 Proteases as a Tool in Food Biotechnology 207
Olga Luisa Tavano

12.1 Introduction 207

12.2 Protease Characteristics 207

12.3 Seeking a More Appropriate Protease 209

12.4 Modifications in Functional and Sensorial Properties of Food Proteins 212

12.5 Cheese-Making 213

12.6 Food Additives 214

12.7 Special Diets 214

12.8 Conclusion 217

References 217

III RECENT ADVANCES IN FERMENTATION TECHNOLOGY 221

13 Application of Metabolic Engineering in Industrial Fermentative Process 223
Mahbuba Rahman

13.1 Introduction 223

13.2 Metabolic Engineering Strategies for Microbial Strain Improvement 224

13.3 Stages and Tools of Metabolic Engineering 225

13.4 Applications of Metabolic Engineering in Fermentation-Based Food Industries 229

13.5 Yeasts 232

13.6 Bacteria 235

13.7 Perspectives 239

References 240

14 Isolation and Selection of Conventional and Non-Conventional Fermentative Yeasts 243
João Simões and Ana Catarina Gomes

14.1 Introduction 243

14.2 Microorganism Relevance in Wine Production 244

14.3 Methods to Recover Fermentative Yeasts 247

14.4 Identification of Fermentative Species 248

14.5 Strain Identification 249

14.6 Fermentative Yeast Phenotypic Characterization 249

14.7 Yeast Improvement Strategies 251

14.8 From the Genome to Phenotype 254

14.9 Future Perspectives and Challenges 256

References 257

15 Multifunctional Lactic Acid Bacteria Cultures to Improve Quality and Nutritional Benefits in Dairy Products 263
Domenico Carminati, Aurora Meucci, Flavio Tidona, Miriam Zago, and Giorgio Giraffa

15.1 Lactic Acid Bacteria: Ecology, Taxonomy and Metabolic Activities 263

15.2 Role of LAB in Dairy Products 265

15.3 LAB Selection and Improvement 268

15.4 Final Remarks 271

References 272

16 New Biotechnological Approaches in Sourdough Bread Production Regarding Starter Culture Applications 277
Stavros Plessas, Ioanna Mantzourani, Argyro Bekatorou, Athanasios Alexopoulos, and Eugenia Bezirtzoglou

16.1 Introduction 277

16.2 Effect of Sourdough on Product Quality 278

16.3 Application of Starter Cultures for Sourdough Bread-Making 278

References 283

17 New Biotechnologies for Wine Fermentation and Ageing 287
Antonio Morata and José A. Suárez-Lepe

17.1 The Return of Non-Saccharomyces Yeasts to Oenology 287

17.2 Influence of Yeasts on Wine Ageing 295

17.3 Future Possibilities 296

17.4 Conclusions 297

References 297

18 Yeast Biotechnology 303
Julie Kellershohn and Inge Russell

18.1 The Market for Yeast and Yeast Products 303

18.2 The Baking Industry 303

18.3 Brewing and Distilling Yeast Developments 304

18.4 Sake Yeast Developments 305

18.5 Wine Production and the Creation of Engineered Malolactic Yeast (ML01) 305

18.6 Food Yeast 305

18.7 Soy Sauce Fermentation 306

18.8 Chymosin for Cheese Production 306

18.9 Flavour Compounds Produced Using Yeast 307

18.10 Carotenoids from Yeast 307

18.11 Saccharomyces Yeast in Non-Food Developments 307

18.12 The Synthetic Yeast Project 307

18.13 The Future 308

References 308

IV FUNCTIONAL FOODS AND NUTRACEUTICALS: NUTRITION, HEALTH AND SAFETY ASPECTS 311

19 Bioencapsulation Technologies for Incorporating Bioactive Components into Functional Foods 313
Kasipathy Kailasapathy

19.1 Health and Functional Foods 313

19.2 Need for Encapsulation 313

19.3 Bioencapsulation Techniques for Administration and Delivery of Bioactive Components 314

19.4 Applications: Encapsulation and Controlled Release of Biofunctional Ingredients in Functional Foods: Selected Examples 320

19.5 Conclusion and Future Trends 328

References 329

20 Gut Microbiota and Polyphenols: A Strict Connection Enhancing Human Health 335
Filomena Nazzaro, Florinda Fratianni, and Antonio d’Acierno

20.1 State of the Art 335

20.2 Polyphenols 337

20.3 Gut Metabotypes and Polyphenols 340

20.4 Influence of Phenolic Compounds on Microbiota Composition 343

20.5 Interaction between Specific Probiotics, Microbiota and Vegetal Sources 344

20.6 Conclusions 345

References 345

21 Improving Probiotics for Functional Foods 351
Lorena Ruiz, Miguel Gueimonde, Patricia Ruas-Madiedo, Abelardo Margolles, and Borja Sánchez

21.1 Introduction 351

21.2 Technological Factors 352

21.3 Physiological Factors 353

21.4 Improving Probiotic Strains I: Strain Selection 354

21.5 Improving Probiotic Strains II: Stress Adaptation 355

21.6 Improving Probiotic Strains III: Strain Production and Food Design 357

21.7 Improving Probiotic Strains IV: Gene Modification 360

21.8 Conclusions and Perspectives 361

Acknowledgements 362

References 362

22 Production of Single-Cell Oil Containing Omega-3 and Omega-6 Fatty Acids 369
Kianoush Khosravi-Darani, Paliz Koohy-Kamaly, Houshang Nikoopour, and Seyedeh Zeinab Asadi

22.1 Introduction 369

22.2 Biochemistry of SCO 370

22.3 Microorganisms Producing SCO 370

22.4 Systems of Cultivation 371

22.5 Commercial Production of SCO 373

22.6 Recovery and Purification of PUFA from SCO 375

22.7 Conclusion 376

References 377

23 Biotechnological Production of Oligosaccharides: Advances and Challenges 381
Diana B. Muñiz-Márquez, Juan C. Contreras, Raúl Rodríguez, Solange I. Mussatto, José A. Teixeira, and Cristóbal N. Aguilar

23.1 Introduction 381

23.2 Beneficial Effects of Oligosaccharides 381

23.3 Types of Oligosaccharides 383

23.4 Other Enzymes used for the Biosynthesis of Oligosaccharides 385

23.5 Microbial Production of Prebiotic Oligosaccharides 386

23.6 Yeast Strains used in Galactooligosaccharide Production from Lactose 386

23.7 Analysis of Oligosaccharides 386

23.8 New Approaches for Purification of Oligosaccharides 387

23.9 Emerging Trends in the Production of Novel Oligosaccharides 388

23.10 Concluding Remarks 388

Acknowledgements 388

References 388

V VALORIZATION OF FOOD WASTE USING BIOTECHNOLOGY 393

24 Biotechnological Exploitation of Brewery Solid Wastes for Recovery or Production of Value-Added Products 395
Argyro Bekatorou, Stavros Plessas, and Ioanna Mantzourani

24.1 Introduction 395

24.2 Generation and Physicochemical Characteristics of Brewery Solid Wastes 397

24.3 Value-Added Bio-Products from Brewery Solid Wastes 399

24.4 Conclusions 408

References 409

25 Value-Added Utilization of Agro-Industrial Residues 415
Sigrid Kusch, Chibuike C. Udenigwe, Cristina Cavinato, Marco Gottardo, and Federico Micolucci

25.1 Introduction 415

25.2 Occurrence and Characteristics of Food Waste 417

25.3 Current and Emerging Food Waste Valorization Strategies 419

25.4 A Spotlight on Functional Foods 421

25.5 Concluding Remarks 424

References 424

26 Cascaded Valorization of Food Waste using Bioconversions as Core Processes 427
Linsey Garcia-Gonzalez, Sebastiaan Bijttebier, Stefan Voorspoels, Maarten Uyttebroek, Kathy Elst, Winnie Dejonghe, Yamini Satyawali, Deepak Pant, Karolien Vanbroekhoven, and Heleen De Wever

26.1 Food Waste: Tomorrow’s Raw Materials? 427

26.2 Characterization of Biomass on a Molecular Level 428

26.3 Extraction of High-Value Compounds 430

26.4 Bioconversions of Food Waste using Enzyme Technology 431

26.5 Bioconversions of Food Waste using Fermentation Technology 433

26.6 Electricity Generation using Microbial Fuel Cells 434

26.7 Conclusions 436

Acknowledgements 436

References 437

27 Potential of Fruits Processing Wastes for Fungal Production of Multi-Enzymes Complexes 443
A.B. Díaz, I. Caro, I. de Ory, and A. Blandino

27.1 Food Processing Wastes as Substrates for SSF 443

27.2 Hydrolytic Enzymes Production from Fruit-Processing Wastes 445

27.3 SSF on Fruit-Processing Wastes in Bioreactors 447

27.4 Application of Hydrolytic Multi-Enzyme Complexes 449

27.5 Use of Enzyme Immobilization Strategies 450

27.6 Conclusions 451

References 451

VI FOOD SAFETY: DETECTION AND CONTROL OF FOOD-BORNE PATHOGENS 455

28 Emergent Strategies for Detection and Control of Biofilms in Food Processing Environments 457
Heidy M.W. den Besten, Yichen Ding, Tjakko Abee, and Liang Yang

28.1 Introduction 457

28.2 Biofilm-Associated Problems in Food Processing Environments 457

28.3 Biofilm Formation Mechanisms of Major Food Pathogens 457

28.4 Mechanisms of Biofilm Resistance 460

28.5 Novel Approaches for Biofilm Detection 461

28.6 Biofilm Control Strategies in Food Industry 462

28.7 Conclusions 466

Acknowledgements 466

References 466

29 Molecular Methods for the Detection and Characterization of Food-Borne Pathogens 471
Gulam Rusul and Li-Oon Chuah

29.1 Introduction 471

29.2 Molecular Detection and Identification of Food-Borne Pathogens 472

29.3 Molecular Typing Techniques 483

29.4 Criteria to Consider when Choosing a Method 486

29.5 Sample Preparation for the Detection of Food-Borne Pathogens 487

29.6 Conclusions 487

References 488

30 Non-Thermal Food Preservation: Control of Food-Borne Pathogens through Active Food Packaging and Nanotechnology 499
Paula Judith Perez Espitia and Rejane Andrade Batista

30.1 Introduction 499

30.2 Polymeric Matrixes and Methods of Food Packaging 500

30.3 Controlling Food-Borne Pathogens through Active Food Packaging 504

30.4 Nanotechnology for Antimicrobial Food Packaging 506

30.5 Safety Issues 506

Acknowledgements 508

References 508

31 Strategies for Advantageous Antimicrobial Activity by Bacteriocins from Lactic Acid Bacteria: Higher Yield, Enhanced Activity and Successful Application in Foods 511
Myrto-Panagiota Zacharof

31.1 Introduction 511

31.2 Bacteriocin Uses and Demands of a Knowledge-Driven Economy 511

31.3 Strategies for Advantageous Production of Bacteriocins 512

31.4 Synergistic Action of Bacteriocins for Enhanced Activity 517

31.5 Application of Bacteriocins in Foods: Examples and Case Studies 519

31.6 Conclusions 521

References 521

32 The Role of Phages in Food-Borne Pathogen Detection 527
Eoghan Nevin, Aidan Coffey, and Jim O’Mahony

32.1 Introduction 527

32.2 Methods of Phage Detection 527

Acknowledgements 535

References 535

VII EMERGING TECHNIQUES IN FOOD PROCESSING 539

33 Applications of Micro- and Nanofluidics in the Food Industry 541
Fabrizio Sarghini

33.1 Introduction 541

33.2 Physical Bases of Microfluidics 542

33.3 Applications 545

33.4 Basic Microfluidic Devices for Food Analysis and Food Processing 548

33.5 Perspectives and Challenges 559

References 560

34 Atmospheric-Pressure Non-Thermal Plasma Decontamination of Foods 565
N.N. Misra, Annalisa Segat, and P.J. Cullen

34.1 Introduction 565

34.2 NTP Fundamentals 566

34.3 Plasma–Microbiological Interactions 568

34.4 Plasma–Food Interactions 569

34.5 Challenges in NTP Processing of Foods 571

34.6 Conclusions and Future Trends 572

34.7 Acknowledgement 572

References 572

35 Electrochemical Processes During High-Voltage Electric Pulses and their Importance in Food Processing Technology 575
Gintautas Saulis, Raminta Rodaitė-Riševičienė, Viktorija SkaidrutėDainauskaitė, and Rita Saulė

35.1 Introduction 575

35.2 Theoretical Background 576

35.3 Consequences of Electrochemical Processes 578

35.4 Methods of Reducing Electrochemical Reaction Intensity and Reaction Consequences 586

35.5 Conclusion 587

Acknowledgements 587

References 587

36 Microencapsulation in Food Biotechnology by a Spray-Drying Process 593
Berta N. Estevinho and Fernando Rocha

36.1 Introduction 593

36.2 Microencapsulation in Food Biotechnology 594

36.3 Microencapsulation Concepts 599

36.4 Spray-Drying Process 600

36.5 Kinetic Mechanisms of Controlled Release 602

36.6 Conclusions 603

Acknowledgements 603

References 603

37 Nanofibre Encapsulation of Active Ingredients and their Controlled Release 607
Filiz Altay and Nagihan Okutan

37.1 Introduction 607

37.2 Encapsulation by Electrospinning 609

37.3 Applications of Electrospun Nanofibre-Encapsulated Ingredients 611

37.4 Controlled Release from Nanofibres 611

37.5 Conclusion and Future Trends 614

Acknowledgements 614

References 614

38 Applications of Nanobiotechnology in the Food Industry 617
Jamuna Bai Aswathanarayan and Ravishankar Rai V.

38.1 Introduction 617

38.2 Nanobiotechnology in Food Packaging: Improved, Intelligent and Active Packaging 618

38.3 Nanotechnology for Delivery of Bioactives and Nutraceuticals 623

38.4 Nanobiosensors: Detection of Food-Relevant Analytes 625

38.5 Safety and Regulatory Aspects of Nanotechnology Applications 630

38.6 Conclusion 630

References 630

39 Recent Advances in and Applications of Encapsulated Microbial and Non-Microbial Active Agents in Food and Beverage Manufacture 635
Viktor Nedovic,ì Branko Bugarski, Fani Mantzouridou, Adamantini Paraskevopoulou, Eleni Naziri, Thomas Koupantsis, Kata Trifkovic ì, Ivana Drvenica, Bojana Balanč, and Verica Ðord̵evic ì

39.1 Introduction 635

39.2 Microbial Food Culture Encapsulation as a Biotechnological Process Tool 636

39.3 Encapsulation for Enhanced In Vivo Bioactive Compound Bioavailability and Improved Aroma 637

39.4 Food-Specific Materials and Methods/Techniques for Encapsulation 642

39.5 Examples of Encapsulated Cell Technology in Fermentation Processes 649

39.6 Examples of Immobilized Cell Technology in Microbial Production of High-Value Food Ingredients 655

39.7 Examples of Encapsulated Cells/Bioactives in Production of Functional Food Products 659

39.8 Trends in Encapsulation 661

39.9 Future Perspectives 665

Acknowledgements 665

References 666

40 Thermal Processing of Food 681
S. K. Pankaj

40.1 Introduction 681

40.2 Cooking Criteria 684

40.3 Retorts 684

40.4 Control Systems 685

40.5 Process Evaluation 687

40.6 On-Line Retort Control 689

40.7 Novel Technologies 689

40.8 Future Trends 691

References 692

Index 693

ABOUT THE EDITOR
Dr Ravishankar Rai V. is Professor at the Department of Studies in Microbiology, University of Mysore, India.