Wheat Rust Diseases, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1659
Coordonnateur : Periyannan Sambasivam
This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.
Preface…
Table of Contents…
Contributors…
Part I Wheat Rust Surveillance and Genotyping
1. Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
Sajid Ali and David Hodson
2. Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
3. Race Typing of Puccinia striiformis on Wheat
4. Assessment of Aggressiveness of Puccinina striiformis on Wheat
5. Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing
6. Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis
7. Computational Methods for Predicting Effectors in Rust Pathogens
8. Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana
9. Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction
10. Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing
11. Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi
Part III Genetics of Rust Resistance
12. Genetic Analysis of Resistance to Wheat Rusts
13. Advances in Identification and Mapping of Rust Resistance Genes in Wheat
14. Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives
15. Application of Genomic Selection in Breeding Wheat for Rust Resistance
Leonardo Ornella, Juan Manuel González-Camacho, Susanne Dreisigacker, and Jose Crossa
16. Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions
Adnan Riaz and Lee Hickey
Part IV Rust Resistance Gene Isolation and Characterization
17. Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning
18. Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
19. MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
20. Rapid Gene Isolation using MutChromSeq
21. Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies
22. BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
23. Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter
Part V Biocontrol Agents for Rust Management
24. Biocontrol Agents for Controlling Wheat Rust
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Date de parution : 06-2018
Ouvrage de 294 p.
17.8x25.4 cm
Date de parution : 08-2017
Ouvrage de 294 p.
17.8x25.4 cm