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Wheat Rust Diseases, 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1659

Langue : Anglais

Coordonnateur : Periyannan Sambasivam

Couverture de l’ouvrage Wheat Rust Diseases

This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.

Preface…
Table of Contents…

Contributors…

Part I Wheat Rust Surveillance and Genotyping


1.     
Wheat Rust Surveillance: Field Disease Scoring and Sample Collection for Phenotyping and Molecular Genotyping
Sajid Ali and David Hodson

2.     
Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
Vanessa Bueno-Sancho, Daniel C.E. Bunting, Luis J. Yanes, Kentaro Yoshida, and Diane G.O. Saunders

3.     
Race Typing of Puccinia striiformis on Wheat
Mogens S. Hovmøller, Julian Rodriguez-Algaba, Tine Thach, and Chris K. Sørensen

4.     
Assessment of Aggressiveness of Puccinina striiformis on Wheat
Chris K. Sørensen, Tine Thach, and Mogens S. Hovmøller

5.     
Extraction of High Molecular Weight DNA from Fungal Rust Spores for Long Read Sequencing
Benjamin Schwessinger and John P. Rathjen

6.     
Microsatellite Genotyping of the Wheat Yellow Rust Pathogen Puccinia striiformis
Sajid Ali, Muhammad R. Khan, Angelique Gautier, Zahoor A. Swati, and Stephanie Walter

Part II Molecular Pathogenicity

7.     
Computational Methods for Predicting Effectors in Rust Pathogens
Jana Sperschneider, Peter N, Dodds, Jennifer M. Taylor, and Sébastien Duplessis

8.     
Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana
Benjamin Petre, Joe Win, Frank L.H. Menke, and Sophien Kamoun

9.     
Proteome Profiling by 2D-Liquid Chromatography for Wheat-Rust Interaction
Semra Hasançebi

10. 
Investigating Gene Function in Cereal Rust Fungi by Plant-Mediated Virus-Induced Gene Silencing
Vinay Panwar and Guus Bakkeren

11. 
Apoplastic Sugar Extraction and Quantification from Wheat Leaves Infected with Biotrophic Fungi
Veronica Roman-Reyna and John P. Rathjen

Part III Genetics of Rust Resistance 

12. 
Genetic Analysis of Resistance to Wheat Rusts
Caixia Lan, Mandeep Singh Randhawa, Julio Huerta-Espino, and Ravi P. Singh

13. 
Advances in Identification and Mapping of Rust Resistance Genes in Wheat
Urmil Bansal and Harbans Bariana

14. 
Chromosome Engineering Techniques for Targeted Introgression of Rust Resistance from Wild Wheat Relatives
Peng Zhang, Ian S. Dundas, Steven S. Xu, Bernd Friebe, Robert A. McIntosh, and W.J. Raupp

15. 
Application of Genomic Selection in Breeding Wheat for Rust Resistance
Leonardo Ornella, Juan Manuel González-Camacho, Susanne Dreisigacker, and Jose Crossa

16.  Rapid Phenotyping Adult Plant Resistance to Stem Rust in Wheat Grown Under Controlled Conditions
Adnan Riaz and Lee Hickey

Part IV Rust Resistance Gene Isolation and Characterization

17.  Generation of Loss of Function Mutants for Wheat Rust Disease Resistance Gene Cloning
Rohit Mago, Bradley Till, Sambasivam Periyannan, Guotai Yu, Brande B.H. Wulff, and Evans Lagudah

18. 
Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
Guotai Yu, Asyraf Hatta, Sambasivam Periyannan, Evans Lagudah, and Brande B.H. Wulff

19. 
MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
Burkhard Steuernagel, Kamil Witek, Jonathan D.G. Jones, and Brande B.H. Wulff

20. 
Rapid Gene Isolation using MutChromSeq
Burkhard Steuernagel, Jan Vrána, Miroslava Karafiátova, Brande B.H. Wulff, and Jaroslav Doležel

21. 
Rapid Identification of Rust Resistance Genes through Cultivar-Specific de novo Chromosome Assemblies
Anupriya K. Thind, Thomas Wicker, and Simon G. Krattinger

22. 
BSMV-Induced Gene Silencing Assay for Functional Analysis of Wheat Rust Resistance
Li Huang

23. 
Yeast as a Heterologous System to Functionally Characterize a Multiple Rust Resistance Gene which Encodes a Hexose Transporter
Ricky J. Milne, Katherine E. Dibley, and Evans S. Lagudah

Part V Biocontrol Agents for Rust Management

24. 
Biocontrol Agents for Controlling Wheat Rust
Siliang Huang and Fahu Pang

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Date de parution :

Ouvrage de 294 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

100,21 €

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Date de parution :

Ouvrage de 294 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

100,21 €

Ajouter au panier