The Plant Cell Wall (2^nd Ed., 2nd ed. 2020) Methods and Protocols Methods in Molecular Biology Series, Vol. 2149

This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Preface…

Acknowledgments…

Table of Contents…

Contributing Authors…

1. High-Voltage Paper Electrophoresis (HVPE)

Stephen C. Fry

Florence Goubet, Paul Dupree, and Katja Salomon Johansen

3. Capillary Electrophoresis with Detection by Laser-Induced Fluorescence

Andrew Mort and Xiangmei Wu

4. Theory and Practice in Measuring In Vitro Extensibility of Growing Plant Cell Walls

Daniel J. Cosgrove

5. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins using Komagataeibacter Fermentation

Deirdre Mikkelsen, Patricia Lopez-Sanchez, Dongjie Wang, and Michael J. Gidley

6. Plant Tissue Cultures

Anna Kärkönen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda

7. Protoplast Isolation and Manipulation in the Unicellular Model Plant, Penium margaritaceum

David S. Domozych, Eleanore Ritter, Anna Lietz, Berke Tinaz, and Sandra C. Raimundo

8. Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens

Alison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, Virginia Lai, and Arielle M. Chaves

9. Expression of Cell-Wall Modifying Enzymes in Aspen for Improved Lignocellulose Processing

Marta Derba-Maceluch and Ewa J. Mellerowicz

10. Activity and Action of Cell-Wall Transglycanases

Lenka Franková and Stephen C. Fry

11. Screening for Cellulolytic Plant Enzymes using Colorimetric and Fluorescence Methods

Kirsten Krause and Stian Olsen

12. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell Walls

Stefan J. Hill, Tracey J. Bootten, Laurence D. Melton, and Philip J. Harris

13. High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron Microscopy

Yunzhen Zheng, Gang Ning, and Daniel J. Cosgrove

14. Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM)

Tobias Keplinger and Ingo Burgert

15. Raman Imaging of Plant Cell Walls

Batirtze Prats Mateu, Peter Bock, and Notburga Gierlinger

16. Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy

Ricardo M. F. da Costa, William Barrett, José Carli, and Gordon G. Allison

Mary L. Tierney, Li Sun, and David S. Domozych

18. Analysis of Plant Cell Walls using High-Throughput Profiling Techniques with Multivariate Methods

John P. Moore, Yu Gao, Anscha J.J. Zietsman, Jonatan U. Fangel, Johan Trygg, William G.T. Willats, and Melané A. Vivier

19. Vibratome Sectioning of Plant Materials for (Immuno)Cytochemical Staining

Olivier Leroux

20. Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Cell Walls from Plants and Marine Algae

Delphine Duffieux, Susan E. Marcus, J. Paul Knox, and Cécile Hervé

Marisa S. Otegui

22. Contributions to Arabinogalactan Proteins Analysis

Romain Castilleux, Marc Ropitaux, Youssef Manasfi, Sophie Bernard, Maïté Vicré-Gibouin, and Azeddine Driouich

23. In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization, FISH

Mário Luís da Costa, María-Teresa Solís, Pilar S. Testillano, and Sílvia Coimbra

24. Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan Proteins

Reina J. Veenhof and Zoë A. Popper

25. Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of Proteins

David Roujol, Laurent Hoffmann, Hélène San Clemente, Corinne Schmitt-Keichinger, Christophe Ritzenthaler, Vincent Burlat, and Elisabeth Jamet

26. Bioinformatic Identification of Plant Hydroxyproline-Rich Glycoproteins

Xiao Liu, Savannah McKenna, Lonnie R. Welch, and Allan M. Showalter

27. Bioinformatics Analysis of Plant Cell Wall Evolution

Elisabeth Fitzek, Rhiannon Balazic, and Yanbin Yin

28. Automated Glycan Assembly of Plant Cell Wall Oligosaccharides

Fabian Pfrengle

29. Computerized Molecular Modelling of Carbohydrates

Alfred D. French and Glenn P. Johnson