Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition (2^nd Ed., Softcover reprint of the original 2nd ed. 2016) Methods in Molecular Biology Series, Vol. 1391

This volume provides a detailed step-by-step description of protocols for the establishment of in vitro cultures of important medicinal plants, their mass multiplication in controlled environment, and step-wise secondary metabolite analysis. Many of these protocols also provide a basis for in vitro germplasm conservation or cryopreservation of medicinal plant species. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and practical, Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition aims to ensure successful results in the further study of this vital field.

1.            Protocols for In Vitro Mass Multiplication and Analysis of Medicinally Important Phenolics of a Salep Orchid, Satyrium nepalense D.Don ("Salam Mishri")

Shashi B. Babbar and Deepak K. Singh

2.           In vitro Culture and Phytochemical Analysis of Passiflora tenuifila Killip and Passiflora setacea DC  (Passifloraceae)

Jenny Sumara Sozoa, Daniel Cuzziol Cruza, Ana Flavia Paveia, Isadora Medeiros da Costa Pereiraa, Marcia Wolfarta, Fernanda Ramlovb, Daiane dos Santosa, Marcelo Maraschinb and Ana Maria Vianaa

3.       Shoot Tip Meristem Cryopreservation of Hypericum Species

Katarína Bruňáková and Eva Čellárová

4.       Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.)

5.       Assessment of In Vitro Biological Activities Of Rich-anthocyanins Plant Species Based on Plinia cauliflora Study Model

Heloisa S. Pitz, Adriana C.D. Trevisan, Fausto R. Cardoso, Al

6.      Biotechnological Approaches for Biomass and Cardenolides Production in Digitalis purpurea

 Naivy Pérez-Alonso, Borys Chong-Pérez, Alina Capote,

7.       In vitro regeneration of endangered medicinal plant Heliotropium kotschyi (Ramram) - Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, and Asma Abahussain

Arpan R. Modi, Vikas Sharma, Ghanshyam Patil, Amritpal S. Singh, N. Subhash, and Nitish Kumar

9. Panax ginseng Adventitious Root Suspension Culture: Protocol for Biomass Production and Analysis of Ginsenosides by High Pressure Liquid Chromatography

Hosakatte Niranjana Murthy and Kee Yoeup Paek

10.Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production

Pınar Nartop

11.  11. In Vitro Propagation and Conservation of Bacopa monnieri L.

Neelam Sharma, Rakesh Singh, and Ruchir Pandey

12.   Establishment, Culture and Scale-Up of Brugmansia candida Hairy Roots for the Production Of Tropane Alkaloids

13. Micropropagation, Acclimatization, and Greenhouse Culture of Veratr

14.  In vitro propagation of Withania somnifera (L.) Dunal

Pritika Singh, Rupam Guleri and Pratap Kumar Pati

Thelma L. Soriano and Evangelina C. Cangao

16. Production of Gymnemic Acid From Cell Suspension Cultures of Gymnema sylvestre

Praveen Nagella, Vijayalaxmi S. Dandin, and Hosakatte Niranjana Murthy

17.  Scale up of Agrobacterium Rhizogenes Mediated Hairy Root Cultures of Rauwolfia serpentina

Shakti Mehrotra, Vikas Srivastava, Manoj K. Goel, and Arun K. Kukreja

18.   In vitro shoot cultures and analysis of steroidal lactones in Withania coagulans (

Rohit Jain, Sumita Kachhwaha, and S.L. Kothari

19. In vitro Propagation of Cannabis sativa L. and Evaluation of Regenerated Plants for Genetic Fidelity and Cannabinoids Content for the Quality Assurance

20. Transcript Quantification of Genes Involved in Steviol Glycoside Biosynthesis in Stevia

21.  In Vitro Propagation and Conservation of Withania somnifera (Dunal) L.

Nigar Fatima, Naseem Ahmad, and Mohammad Anis

22.  Construction of Hypericin Glands Specific cDNA Library via Suppression Subtractive Hybridization

Rupesh Kumar Singh, Weina Hou, and G. Franklin

Ritu Mahajan

Jaykumar J. Chavan and Mahendra L. Ahire

25. Somatic Embryogenesis of Date Palm (Phoenix dactylifera L.) through Cell Suspension Culture

Poornananda M. Nai, and  Jameel M. Al-Khayri

26.   Protocols for Improvement Of Black Pepper Utilizing Biotechnological Tools

K. Nirmal Babu , Divakaran Minoo, G. Yamuna ,P.N. Ravindran,  and K.V.Peter

27.   Protocols for In Vitro Propagation, Conservation, Synthetic Seed Production, Microrhizome Production and Molecular Profiling in Turmeric (Curcuma longa L.)

28.   In Vitro Propagation, and Genetic Transformation In Ginger (Zingiber officinale Roscoe.) 

29.   Hairy Root Cultures of  Gymnema sylvestre

30.   Bioreactors for Mass Propagation of Cymbopogon citratus Sapf. 

 Elisa Quiala, Raúl Barbón, Alina Capote, Naivy Pérez, and Elio Jiménez

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts