Plant Senescence, 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1744

Part I: Introduction of Plant Senescence

1. Concepts and Types of Senescence in Plants

            Susheng Gan

2. Initiation, Progression, and Genetic Manipulation of Leaf Senescence

            Akhtar Ali, Xiaoming Gao, and Yongfeng Guo

Part II: Phenotypic Analysis and Molecular Markers of Plant Organ Senescence

3. Phenotypic Analysis and Molecular Markers of Leaf Senescence

4. Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose

            Chenxia Cheng, Junping Gao, and Nan Ma

5. Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence

            Xiuying Xia

6. Quantitative Analysis of Floral Organ Abscission in Arabidopsis via a Petal Breakstrength Assay

            Chun-Lin Shi and Melinka A. Butenko

7. Characterization of Climacteric and Non-Climacteric Fruit Ripening

Part III: Hormonal Control of Plant Senescence

8. Ethylene Treatment in Studying Leaf Senescence in Arabidopsis

            Zhonghai Li and Hongwei Guo

9. The Assay of Abscisic Acid-Induced Stomata Movement in Leaf Senescence

            Yanyan Zhang and Kewei Zhang

10. The EPR Method for Detecting Nitric Oxide in Plant Senescence

            Aizhen Sun

11. Hormone Treatments in Studying Leaf Senescence

            Zenglin Zhang and Yongfeng Guo

Part IV: Stress-Induced Senescence in Plants

12. Methods to Study Darkness-Induced Leaf Senescence

            Yi Song and Lin Li

13. Salt Treatments and Induction of Senescence

            Yasuhito Sakuraba, Dami Kim, and Nam-Chon Paek

14. Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance

            Shoki Aoyama, Junji Yamaguchi, and Takeo Sato

15. Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection

            Wei Liu, Wenwei Zhang, Na Zheng, Weibo Zhai, and Fangjun Qi

16. Study of Hydrogen Peroxide as a Senescence-Inducing Signal

            Stefan Bieker, Maren Potschin, and Ulrike Zentgraf

17. Identification of Postharvest Senescence Regulators through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue

            Donald A. Hunter, Rubina Jibran, Paul Dijkwel, David Chagné, Kerry Sullivan, Aakansha Kanojia, and Ross Crowhurst

Part V: Molecular and Cellular Processes in Plant Senescence

18. Chlorophyll and Chlorophyll Catabolite Analysis by HPLC

            Aditi Das, Luzia Guyer, and Stefan Hörtensteiner

19. Identification and Functional Analysis of Senescence-Associated Genes in Wheat

            Geng Wang, Ke Li, and Chunjiang Zhou

20. Isolation, Purification, and Detection of MicroRNAs in Plant Senescence

            Yujun Ren and Ying Miao

21. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay

            Branka Uzelac, Dušica Janošević, and Snežana Budimir

22. Activities of Vacuolar Cysteine Proteases in Plant Senescence

            Dana E Martínez, Lorenza Costa, and Juan José Guiamét

23. Study of Autophagy in Plant Senescence

            Xuefei Cui, Jing Zheng, Jinxin Zheng, and Qingqiu Gong

24. Isolation of ChloroplastsforIn Organelle Protein Degradation Assay

            Scott W. Vande Wetering and Judy A. Brusslan

25. Plant Cell Walls: Isolation and Monosaccharide Composition Analysis

            Yingzhen Kong, Malcolm O’Neill, and Gongke Zhou

26. Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope

            Chun-Lin Shi and Melinka A. Butenko

Part VI: Systems Biology in Plant Senescence

27. RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco

            Wei Li and Yongfeng Guo

28. Comprehensive Metabolomics Studies of Plant Developmental Senescence

Includes cutting-edge methods and protocols for the study of senescence in plants

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts