Plant Receptor Kinases, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1621

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PART I Expression and Purification of PRKs

1. Expression of Plant Receptor Kinases in E. coli 

2. Expression of Plant Receptor Kinases in Yeast

María Laura Barberini and Jorge Muschietti

3. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells

Hidefumi Shinohara and Yoshikatsu Matsubayashi

4. Cell-free Synthesis of Plant Receptor Kinases

5. Purification of Plant Receptor Kinases from Plant Plasma Membranes

Jin Suk Lee

PART II Ligand Binding and Activation of PRKs

6. Photoaffinity Labeling of Plant Receptor Kinases

Hidefumi Shinohara and Yoshikatsu Matsubayashi

7. Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases

Markus Albertand Ursula Fürst

PART III In silico Methods for Identification and Analyses of PRKs

8. Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis

Aleksia Vaattovaara, Jarkko Salojärvi, and Michael Wrzaczek

9. In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping

Russell J. S. Orr and Reidunn B. Aalen

PART IV Identification of Enzymatic Functions and Interacting Partners of PRKs

10. Co-immunoprecipitation of Plant Receptor Kinases

11. Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems

Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, and Tatsuya Sawasaki

12. Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners

Matthew R Meyer, Shweta Shah, and A. Gururaj Rao

13. In vitro Assessment of Guanylyl Cyclase Activity of Plant Receptor Kinases

Misjudeen Raji and Chris Gehring

14. BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases

Cornelia Fischer, Margret Sauter, and Petra Dietrich

15. Pull Down Assay to Characterize Ca²⁺/Calmodulin Binding to Plant Receptor Kinases

Christine Kaufmann and Margret Sauter

PART V Imaging of PRK Dynamics

Stefanie Weidtkamp-Peters and Yvonne Stahl

17. Detection and Analyses of Endocytosis of Plant Receptor Kinases

Includes cutting-edge methods and protocols

Provides step-by-step detail essential fro reproducible methods

Contains key notes and implementation advice from the experts