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Plant molecular biology manual, 2nd ed including supplement 4, 2000 (2 volumes) (2nd Ed., 2nd ed. 2000. Softcover reprint of the original 2nd ed. 2000)

Langue : Anglais

Coordonnateurs : Gelvin S.B., Schilperoort R.A.

Couverture de l’ouvrage Plant molecular biology manual, 2nd ed including supplement 4, 2000 (2 volumes)
Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition. The Second Edition includes Supplement III and further Supplements will be published regularly.
Supplement I : F4. Polymerase chain reaction: gene detection, inverse PCR, and genetic engineering, J. Slightom, et al. G3. Light microscopic applications of immunocytochemistry, K. Vandenbosch, et al. K3. T-DNA tagging in Arabidopsis thaliana: Cloning by gene disruption, B. Schulz, et al. L1. High level gene expression in plants using RNA viruses as transient expression vectors, C.M. Kearney, et al. L2. Geminivirus mediated gene transfer, D. Bisaro, G. Sunter. L3. Caulimovirus mediated gene transfer, I. Jupin, B. Gronenborn. Supplement II : RNA differential display, M.J. Laskowski. Expression analysis of multigene Families by RFLP-coupled domain-directed differential display (RC4D), A. Fischer, et al. Recombinant aequorin methods for measurement of intracellular calcium in plants, H. Knight, et al. GUS activity staining -- A powerful tool in plant molecular biology, Jeong Hee Lee, F. Schoffl. In-Cell RT-PCR in a single, detached plant cell, Ho-Hyung Woo. Supplement III : A3. Liposome-mediated transfer of YAC-DNA to tobacco cells, M. Van Wordragen, et al. B7. Agribacterium germ-line transformation: Transformation of Arabidopsis without tissue culture, A.F. Bent, S. Clough. D6. RNA stability of chloroplasts, J. Nickelsen. G5. Preparation of extended DNA fibres for high resolution mapping by fluorescence in situ hybridization (FISH), P. Fransz, et al. L4. Tobamovirus transient expression vectors: Tools for plant biology and high-level expression of foreign proteins in plants, G.P. pogue, et al. M1. Identification and characterization of protein interactions using the yeast 2-hybrid system, S.E. Kohalmi, et al. Index.,
This manual contains both fundamental and recently described techniques in the area of plant molecular biology. Designed for the use in the research laboratory, it presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection sy

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