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Plant Metabolic Engineering, 1st ed. 2022 Methods and Protocols Methods in Molecular Biology Series, Vol. 2396

Langue : Anglais

Coordonnateur : Shulaev Vladimir

Couverture de l’ouvrage Plant Metabolic Engineering

This volume looks at the latest techniques used by researchers to study various aspects of plant metabolic engineering. The chapters in this book cover topics such as bioinformatics tools used to discover new genes and pathways; heterologous expression of biosynthetic genes in plant and microbial systems; and omics technologies, such as transcriptomics, proteomics, metabolomics, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Plant Metabolic Engineering: Methods and Protocols is a valuable resource for biologists, chemists, biotechnologists, students, and broad cohorts of researchers who works in the fields of plant metabolism and metabolic engineering. 


Preface…
Table of Contents…
Contributing Authors…

1. Methods for the Development of Recombinant Microorganisms for the Production of Natural Products 
Alexander Perl, YeJong Yoo, Hunter Dalton, and Mattheos A. G. Koffas

2. Sustainable Technological Methods for the Extraction of Phytochemicals from Citrus Byproducts
Salvatore Multari, Fulvio Mattivi, and Stefan Martens

3. Reconstitution of Metabolic Pathway in Nicotiana benthamiana
Chenggang Liu

4. A Protocol for Phylogenetic Reconstruction 
Soham Sengupta and Rajeev K. Azad

5. RNA-Seq Data Analysis Pipeline for Plants: Transcriptome Assembly, Alignment, and Differential Expression Analysis
David J. Burks and Rajeev K. Azad

6. A Protocol for Horizontally Acquired Metabolic Gene Detection in Algae
Ravi S. Pandey and Rajeev K. Azad

7. Global Comparative Label-Free Yeast Proteome Analysis by LC-MS/MS After High-pH Reversed-Phase Peptide Fractionation Using Solid-Phase Extraction Cartridges
Khadiza Zaman, Prajita Pandey, Vladimir Shulaev, and Laszlo Prokai

8. Gas Chromatography Coupled to Atmospheric Pressure Chemical Ionization
High Resolution Mass Spectrometry for Metabolite Fingerprinting of Grape (Vitis vinifera L.) Berry
Johana S. Revel, Armando Alcazar Magana, Jeffrey Morré, Laurent Deluc, and Claudia S. Maier

9. GC-MS/MS Profiling of Plant Metabolites
Feroza Kaneez Choudhury, Prajita Pandey, Ron Mittler, Dwain Cardona, Amit C. Gujar, and Vladimir Shulaev

10. Analysis of Grape Volatiles using Atmospheric Pressure Ionization Gas Chromatography Mass Spectrometry-Based Metabolomics
Manoj Ghaste, Fulvio Mattivi, Giuseppe Astarita, and Vladimir Shulaev

11. A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites 
Giuseppe Paglia and Giuseppe Astarita

12. Macrolipidomic Profiling of Vegetable Oils: The Analysis of Sunflower Oils with Different Oleic Acid Content
Juan J. Aristizabal-Henao and Ken D. Stark

13. Non-Targeted Lipidomics using a Robust and Reproducible Lipid Separation using UPLC with Charged Surface Hybrid Technology and High Resolution Mass Spectrometry
Giorgis Isaac, Vladimir Shulaev, and Robert S. Plumb

14. Comprehensive Analysis of Plant Lipids using Sub-2µm Particle CO2-Based Chromatography Coupled to Mass Spectrometry
Carolina Salazar, Michael D. Jones, Giorgis Isaac, and Vladimir Shulaev

15. Bioinformatics in Lipidomics: Automating Large-Scale LC-MS-Based Untargeted Lipidomics Profiling with SimLipid Software
Ningombam Sanjib Meitei and Vladimir Shulaev

16. A Protocol for Prion Discovery in Plants 
Jamie D. Dixson and Rajeev K. Azad

17. Structural Determination of Uridine Diphosphate Glycosyltransferases using X-Ray Crystallography
Kasandra Alderete and Xiaoqiang Wang

Subject Index List…

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts

Date de parution :

Ouvrage de 244 p.

17.8x25.4 cm

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137,14 €

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Date de parution :

Ouvrage de 244 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

189,89 €

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Thème de Plant Metabolic Engineering :

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