Preface…
Table of Contents…
Contributing Authors…
1 Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains
Jay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, and Kee Hoon Sohn
2 Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine- Acetylated Proteins Expressed in planta
Maxime Escouboué and Laurent Deslandes
3 Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl CoenzymeA
Karl J. Schreiber and Jennifer D. Lewis
4 A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions
Mustafa Adhab and James E. Schoelz
5 Quantification of Extracellular ATP in Plant Suspension Cell Cultures
Sowmya R. Ramachandran, Sonika Kumar, and Kiwamu Tanaka
6 Measuring Pectin Properties to Track Cell Wall Alterations during Plant-Pathogen Interactions
Gerit Bethke and Jane Glazebrook
7 Method to Study Dynamics of Membrane-Bound Plant Transcription Factors during Biotic Interactions in Tomato
Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, and Pallob Kundu
8 Preparation of Plant Material for Analysis of Protein-Nucleic Acid Interactions by FRET- FLIM
Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, and Laurent Deslandes
9 Analysis of DNA Methylation Profile in Plants by Chop-PCR
Pratiti Dasgupta and Shubho Chaudhuri
10 Change in Nucleosome Dynamics during Stress Responses in Plants
Amit Paul and Shubho Chaudhuri
11 Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry
Leiyun Yang, Zhixue Wang, and Jian Hua
12 A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem under Various Light Environments
Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, and Emmanuel Liscum
13 Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae
Conner J. Rogan and Jeffrey C. Anderson
14 Quantification of Cauline Leaf Abscission in Response to Plant Pathogens
O. Rahul Patharkar
15 Methods for Replicating Leaf Vibrations Induced by Insect Herbivores
Sabrina C.J. Michael, Heidi A. Appel, and Reginald B. Cocroft
16 Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes
Marieke van Hulten, Sayantani Chatterjee, and Harrold A. van den Burg
17 The Apple Fruitlet Model System for Fire Blight Disease
Sara M. Klee, Judith P. Sinn, and Timothy W. McNellis
18 Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins
Sharon Pike, Walter Gassmann, and Jianbin Su
19 Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems
Ankita Shrestha, Ahamed Khan, and Nrisingha Dey
20 Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants
Ahamed Khan, Ankita Shrestha, and Nrisingha Dey
21 Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth
Robert J. Kremer
22 A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance
Eduardo Balsanelli, Vânia Carla Pankievicz, Valter Antonio Baura, Fábio de Oliveira Pedrosa, and Emanuel Maltempi de Souza
Subject Index List…