Plant Cell Division, 1st ed. 2016 Methods and Protocols Methods in Molecular Biology Series, Vol. 1370

This volume aims to present a large panel of techniques for the study of Plant Cell Division. Plant Cell Division: Methods and Protocols captures basic experimental protocols that are commonly used to study plant cell division processes, as well as more innovative procedures. Chapters are split into five parts covering several different aspect of plant cell division such as,   cell cultures for cell division studies, cell cycle progression and mitosis, imaging plant cell division, cell division and morphogenesis, and cytokinesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Division: Methods and Protocols is a valuable tool for the study of plant cell division at both the cellular and molecular levels, and in the context of plant development.

Part I: Cell Cultures for Cell Division Studies

1.      Synchronization Of Green Algae By Light And Dark Regimes For Cell Cycle and Cell Division Studies

 Monika Hlavová, Milada Vítová, and Kateřina Bišová

2.      Plant Cell Division Analyzed by Transient Agrobacterium-Mediated Transformation of Tobacco BY-2 Cells

 Henrik Buschmann

Part II: Cell Cycle and Mitosis

3.      Identifying Gene Regulatory Networks In Arabidopsis By In Silico Prediction, Yeast-1-Hybrid and Inducible Gene Profiling Assays

 Erin E. Sparks and Philip N. Benfey

4.      Cytrap Marker Systems for In Vivo Visualization of Cell Cycle Progression in Arabidopsis

 Shiori Sugamata Aki and Masaaki Umeda

5.      Imaging Nuclear Morphology and Organization in Cleared Plant Tissues Treated with Cell Cycle Inhibitors

 José Dijair Antonino De Souza Junior, Maria Fatima Grossi De Sa, Gilbert Engler and,  Janice De Almeida Engler

6.      Isolation of Plant Nuclei at Defined Cell Cycle Stages Using Edu Labeling and Flow Cytometry

 Emily E. Wear, Lorenzo Concia, Ashley M. Brooks, Emily A. Markham, Tae-Jin Lee, George C. Allen, William F. Thompson,and Linda Hanley-Bowdoin

7.      Preparation and Fluorescent Analysis of Plant Metaphase Chromosomes

 Trude Schwarzacher

8.      Fluorescence In Situ Hybridization On Rice Chromosomes

 Yafei Li and Zhukuan Cheng

Part III Imaging Plant Cell Division

9.      Dynamics of the Plant Nuclear Envelope During Cell Division

 David E Evans and Katja Graumann

10.  Immunolabeling Of Nuclei/Chromosomes In Arabidopsis Thaliana

Michael Sandmann, Joerg Fuchs, and Inna Lermontova

11.  In Vivo Imaging Of Microtubules Organization In Dividing Giant Cell

 Marie-Cecile Caillaud and Bruno Favery

Part IV: Cell Division and Morphogenesis

12.  Real-Time Lineage Analysis to Study Cell Division Orientation in the Arabidopsis Shoot Meristem

13.  Cell Proliferation Analysis Using Edu Labeling In Whole Plant and Histological Samples Of Arabidopsis

Anita Kazda, Svetlana Akimcheva, J. Matthew Watson, and Karel Riha

14.  Studying Cell Division Plane Positioning in Early-Stage Embryos

 Katia Belcram, Jean-Christophe Palauqui, and Martine Pastuglia

Part V: Cytokinesis

15.  Characterization Oof Cytokinetic Mutants using Small Fluorescent Probes

 Andrei Smertenko, Panagiotis Moschou, Laining Zhang, Deirdre Fahy, and Peter Bozhkov

16.  Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing Carolyn G. Rasmussen

 Patricia Gerbeau-Pissot, Christophe Der, Markus Grebe, and Thomas Stanislas

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts