Photosynthesis, Softcover reprint of the original 1st ed. 2018 Methods and Protocols Methods in Molecular Biology Series, Vol. 1770

Langue : Anglais

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This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.

Part I: Measuring Photosynthetic Parameters

1. Survey of Tools for Measuring In VivoPhotosynthesis

Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, and Tracy Lawson

2. Photosynthetic Gas Exchange in Land Plants at the Leaf Level

Florian A. Busch

3. Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants

Gavin M. George, Katharina Kölling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flütsch, and Samuel C. Zeeman

4. Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)

Qingfeng Song and Xin-Guang Zhu

5. Light-Response Curves in Land Plants

Robert A. Coe and HsiangChun Lin

6. Chlorophyll Fluorescence on the Fast Timescale

Olubukola O. Ajigboye, Rumiana V. Ray, and Erik H. Murchie

7. Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield

Thomas J. Avenson and Aaron J. Saathoff

8. Chlorophyll Fluorescence Imaging

Tracy Lawson and Silvere Vialet-Chabrand

9. Measurement of O₂ Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry

Steven M. Driever and Neil R. Baker

10. Using Carbon Stable Isotopes to Study C₃ and C₄ Photosynthesis: Models and Calculations

Nerea Ubierna, Meisha-Marika Holloway-Phillips, and Graham D. Farquhar

11. Liquid-Phase Measurements of Photosynthetic Oxygen Evolution

Dmitriy Shevela, Wolfgang P. Schröder, and Johannes Messinger

Part II: Measuring Photosynthetic Enzyme Abundance and Catalytic Activity

12. Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting

J. Alejandro Perdomo, Cristina R.G. Sales, and Elizabete Carmo-Silva

13. Extraction of Rubisco to Determine Catalytic Constants

Douglas J. Orr and Elizabete Carmo-Silva

14. Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts

Cristina R.G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, and Elizabete Carmo-Silva

Part III: Determining Cellular and Sub-Cellular Phenotypes

15. Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features

Roxana Khoshravesh and Tammy L. Sage

16. Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves

Vivien Rolland

17. 3D Clearing and Molecular Labeling in Plant Tissues

William M. Palmer, Jamie R. Flynn, Antony P. Martin, Stephanie L. Reed, Christopher P.L. Grof, Rosemary G. White, and Robert T. Furbank

18. Evaluation of Lipids for the Study of Photosynthetic Membranes

Helmut Kirchhoff and Robert Yarbrough

Part IV: Photosynthesis-Inspired Energy Generation

19. “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs

Jackson D. Megiatto Jr. and Catia Ornelas

20. Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation

Stephen J.L. Rowden, Paolo Bombelli, and Christopher J. Howe

Includes cutting-edge methods and protocols for the study of photosynthesis

Provides step-by-step detail essential for the best results

Contains key notes and implementation advice from the experts