Photorespiration, Softcover reprint of the original 1st ed. 2017 Methods and Protocols Methods in Molecular Biology Series, Vol. 1653

This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Estimation of Photorespiratory Fluxes by Gas Exchange

Florian A. Busch, Ross M. Deans, and Meisha-Marika Holloway-Phillips

2. Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling

Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, and Frank Van Breusegem

3. Measurement of Enzyme Activities

Hermann Bauwe

4. In vitro Alkylation Methods for Assessing the Protein Redox State

Flavien Zannini, Jérémy Couturier, Olivier Keech, and Nicolas Rouhier

5. Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants

 Ines Lassowskat, Markus Hartl, Fabian Hosp, Paul J. Boersema, Matthias Mann, and  Iris Finkemeier

6. In vitro Analysis of Metabolite Transport Proteins

Marc-Sven Roell, Franziska Kuhnert, Shirin Zamani-Nour, and Andreas P.M. W

Stéphanie Arrivault and Toshihiro Obata

8. Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration

Stefan Timm, Katharina Modde, and Hermann Bauwe

9. Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits

Franklin Magnum de Oliveira Silva, Lucas de Ávila Silva, Wagner L. Araújo, Agustin Zsögön, and Adriano Nunes-Nesi

10. Metabolic Engineering of Photorespiration

Martin KM Engqvist and Veronica G Maurino

11. ¹³CO₂ Labelling and Mass Spectral Analysis of Photorespiration

Leonardo Perez de Souza, Marek Szecówka, Alisdair R. Fernie, and Takayuki Tohge

12. Isotopically Nonstationary Metabolic Flux Analysis (INST-M

Fangfang Ma, Lara J. Jazmin, Jamey D. Young, and Doug K. Allen

13. Genome-scale Modeling of Photorespiratory Pathway Manipulation

14. Kinetic Modelling of Photorespiration

Honglong Zhao, Yi Xiao, and Xin-Guang Zhu

15. Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues

Alisdair R. Fernie, Hermann Bauwe, and Lee J. Sweetlove

16. Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana

Stephan Krueger, Ruben M. Benstein, Sabine Wulfert, Armand D. Anoman, María Flores-Tornero, and Roc Ros

17. Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration

Roxana Khoshravesh, Vanessa Lundsgaard-Nielsen, Stefanie Sultmanis, and Ta

Includes cutting-edge and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras