Molecular Plant Taxonomy (2^nd Ed., 2nd ed. 2021) Methods and Protocols Methods in Molecular Biology Series, Vol. 2222

1. Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives

            Germinal Rouhan and Myriam Gaudeul

2. Guidelines for the Choice of Sequences for Molecular Plant Taxonomy

            Pascale Besse

3. Isolation and Purification of DNA from Complicated Biological Samples

            Ruslan Kalendar, Svetlana Boronnikova, and Mervi Seppänen

4. Herbarium Specimens: A Treasure for DNA Extraction, an Update

            Lenka Záveská Drábková

5. Sequencing of Complete Chloroplast Genomes

            Berthold Heinze

6. Utility of the Mitochondrial Genome in Plant Taxonomic Studies

            Jérôme Duminil and Guillaume Besnard

7. Nuclear Ribosomal RNA Genes: ITS Region

            Pascale Besse

8. Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla

            Pascale Besse, Denis Da Silva, and Michel Grisoni

9. High-Throughput Genotyping Technologies in Plant Taxonomy

10. Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny

            Félicien Favre, Cyril Jourda, Pascale Besse, and Carine Charron

11. Development of Microsatellite Markers Using Next-Generation Sequencing

            Hélène Vignes and Ronan Rivallan

12. Amplified Fragment Length Polymorphism: Applications and Recent Developments

            Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, and Kantipudi Nirmal Babu

13. Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques

            Kantipudi Nirmal Babu, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby, and Illathidath Payatatti Vijesh Kumar

14. Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers

            Chrissen E.C. Gemmill and Ella R.P. Grierson

15. Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity

            Ruslan Kalendar, Alexander Muterko, and Svetlana Boronnikova

16. Introduction to Population Genomics Methods

            Thibault Leroy and Quentin Rougemont

17. The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants

            Jaume Pellicer, Robin F. Powell, and Ilia J. Leitch

18. Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization

            Sonja Siljak-Yakovlev, Fatima Pustahija, Vedrana Vicic Bockor, and Odile Robin

19. GISH: Resolving Interspecific and Intergeneric Hybrids

            Nathalie Piperidis

Includes cutting-edge techniques

Provides step-by-step detail essential for reproducible results

Contains key implementation advice from the experts