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G Protein-Coupled Receptor Signaling in Plants, Softcover reprint of the original 1st ed. 2013 Methods and Protocols Methods in Molecular Biology Series, Vol. 1043

Langue : Anglais

Coordonnateur : Running Mark P.

Couverture de l’ouvrage G Protein-Coupled Receptor Signaling in Plants

Remarkably, while G protein-coupled receptors (GPCRs) are highly prevalent in animals and yeast, very few candidate GPCRs have been identified in plants. In G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols, experts in the field describe techniques used in the study of small GTPases and related proteins.  Beginning with a chapter on bioinformatics approaches for GPCR discovery, this detailed volume continues with chapters on heterotrimeric G protein subunits, Rab-GTPases, as well as lipid modifications, including myristoylation, acylation, and prenylation.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and dependable, G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols aims to aid further studies into the roles of small GTPases which will help elucidate numerous key processes in plants.

1. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

            Timothy E. Gookin and Jannick D. Bendtsen

 

2. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

            Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, and Sona Pandey

 

3. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

            Jian Zhao and Xuemin Wang

 

4. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

            Zhaoqing Jin, Wellington Muchero, and Jin-Gui Chen

 

5. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis

            Kiwamu Tanaka, Jeongmin Choi, and Gary Stacey

 

6. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis

            Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, and Yiji Xia

 

7. Fusarium oxysporum Infection Assays in Arabidopsis

            Yuri Trusov, David Chakravorty, and Jose Ramon Botella

 

8. Analysis of Unfolded Protein Response in Arabidopsis

            Yani Chen and Federica Brandizzi

 

9. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis

            Wenjuan Wu and Jirong Huang

 

10. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings

            Danielle A. Orozco-Nunnelly, Lon S. Kaufman, and Katherine M. Warpeha

 

11. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana

            Xingyun Qi and Huanquan Zheng

 

12. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue

            Myoung Hui Lee, Yongjik Lee, and Inhwan Hwang

 

13. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography–Coupled Mass Spectrometry

            Nadav Sorek, Amir Akerman, and Shaul Yalovsky

 

14. In Vitro Myristoylation Assay of Arabidopsis Proteins

            Xuehui Feng, Wan Shi, Xuejun Wang, and Mark P. Running

 

15. Assaying Protein S-Acylation in Plants

            Piers A. Hemsley

 

16. In Vitro Prenylation Assay of Arabidopsis Proteins

            Wan Shi, Qin Zeng, and Mark P. Running

Explores techniques to examine the roles of GCRPs and small GTPases in key plant biological processes

Features step-by-step protocols that are ready for the lab

Includes expert tips and key implementation advice to ensure quality results

Date de parution :

Ouvrage de 162 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

110,76 €

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Date de parution :

Ouvrage de 162 p.

17.8x25.4 cm

Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).

105,49 €

Ajouter au panier