Chloroplast Biotechnology, 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1132

In Chloroplast Biotechnology: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used in chloroplast molecular biology. Chapters focus on essential background information, applications in tobacco and protocols for plastid transformation in crops and Chlamydomonas and Bryophytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoidance of known pitfalls.

Authoritative and practical,  Chloroplast Biotechnology: Methods and Protocols seek to aid scientists who study chloroplast molecular biology as well as those interested in applications in agriculture, industrial biotechnology and healthcare.

Part I:  Background

1. The Plastid Genomes of Flowering Plants

Tracey A. Ruhlman and Robert K. Jansen

2. Next Generation Technologies to Determine Plastid Genome Sequences

Robert J. Henry, Nicole Rice, Mark Edwards and Catherine J. Nock

3. Plastid Gene Transcription: Promoters and RNA Polymerases

Jennifer Ortelt and Gerhard Link

4. Plastid mRNA Translation

Masahiro Sugiura

5. Engineering Chloroplasts for High-Level Foreign Protein Expression

Ralph Bock

6. Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences

Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila and Anil Day

7. Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-Green Plastids

Maureen R. Hanson and Amirali Sattarzadeh

Part II: Nicotiana Tabacum the Model Species of Chloroplast Biotechnology

8. Plastid Transformation in Nicotiana tabacum and Nicotiana sylvestris by Biolistic DNA Delivery to Leaves

Pal Maliga and Tarinee Tungsuchat Huang

9. Nicotiana tabacum – PEG-mediated Plastid Transformation

Areli Herrera Díaz and Hans-Ulrich Koop

10. Plastid Transformation of Tobacco Suspension Cell Cultures

Jeffrey M. Staub

11. Tryptophan and Indole Analog Mediated Plastid Transformation

Pierluigi Barone,  Xing-Hai Zhang and Jack M. Widholm

12. Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre Recombinase

Tarinee Tungsuchat-Huang and Pal Maliga

13. Determination of the Half-Life of Chloroplast Transcripts in Tobacco Leaves

Sithichoke Tangphatsornruang and John C. Gray

14. Quantification of Organellar DNA and RNA Using Real-Time PCR

Andreas Weihe

15. Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expression

Spencer M. Whitney and Robert E. Sharwood

Part III: Crop Specific Plastid Transformation Protocols

16. Plastid Transformation in Tomato

Stephanie Ruf and Ralph Bock

17. Stable Plastid Transformation of Petunia

Elena Martin Avila and Anil Day

18. Plastid Transformation in Potato - Solanum tuberosum

Vladimir T. Valkov, Daniela Gargano, Nunzia Scotti, and Teodoro Cardi

19. Plastid Transformation in Eggplant

Kailash C. Bansal and Ajay K. Singh

Cilia L.C. Lelivelt, Kees M.P. van Dun, C. Bastiaan de Snoo, Matthew S. McCabe, Bridget V. Hogg, and Jacqueline M. Nugent

21. Plastid Transformation in Lettuce (Lactuca sativa L)by Biolistic DNA Delivery

Tracey A. Ruhlman

22. Plastid Transformation in Soybean

Manuel Dubald, Ghislaine Tissot and Bernard Pelissier

23. Plastid Transformation in Cabbage (Brassica oleracea L. var. capitata L.) by the Biolistic Process

Menq-Jiau Tseng, Ming-Te Yang, Wan-Ru Chu, and Cheng-Wei Liu

24. Plastid Transformation in Sugar Beet – Beta vulgaris

Francesca De Marchis and Michele Bellucci

25. Integration and Expression of gfp in the Plastid of Medicago sativa L.

Shaochen Xing, Zhengyi Wei, Yunpeng Wang, Yanzhi Liu, Chunjing Lin

Part IV: Plastid Transformation in Algae

26. Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids

Daniel Barrera, Javier Gimpel and Stephen Mayfield

27. A Simple, Low Cost Method for Chloroplast Transformation of the Green Alga Chlamydomonas reinhardtii

Chloe Economou, Thanyanan Wannathong, Joanna Szaub and Saul Purton

28. Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas

Jean-David Rochaix, Raymond Surzycki and Silvia Ramundo

Part V : Plastid Transformation in Bryophyte

29. Plastid Transformation in Physcomitrella patens

Mamoru Sugita

30. Plastid Transformation of Sporelings and Suspension-cultured Cells from the Liverwort Marchantia polymorpha L.

Shota Chiyoda, Katsuyuki T. Yamato, and Takayuki Kohchi

Presents current up-to-date methodologies on chloroplast molecular biology

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras