Barley, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1900

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

An Introduction to Barley: The Crop and the Model.- Mutation Breeding in Barley:  Historical Overview.- A Practical Guide to Barley Crossing.- Barley Anther Culture.- Isolated Microspore Culture in Barley.- TILLING in Barley.- Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings.- Agrobacterium-Mediated Transformation of Barley Immature Embryos.- Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses during Early Development.- Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy.- Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy.- Microarrays for High-Throughput Gene Expression Analysis of Barley.- Genome Engineering Using TALENs.- Creating Targeted Gene Knock-Outs in Barley Using CRISPR/Cas9.- Genotyping-by-Sequencing on the Ion Torrent Platform in Barley.- DNA Methylation Analysis in Barley and Other Species with Large Genomes.- High Resolution RT-PCR Analysis of Alternative Barley Transcripts.- Exome Capture for Variant Discovery and Analysis in Barley.

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key notes and expert implementation advice