Proteomic Profiling (2nd Ed., 2nd ed. 2021) Methods and Protocols Methods in Molecular Biology Series, Vol. 2261
1. How Modern Mass Spectrometry Can Solve Ancient Questions: A multi-Omics Study of the Stomach Content of the Oldest Human Ice Mummy, the 5300-Year-Old Iceman or Oetzi
Rudolf Grimm
2. Step-by-Step Sample Preparation of Proteins for Mass Spectrometric Analysis
Xinping Li, Thomas Franz, Ilian Atanassov, and Thomas Colby
3. Lab-on-a-Filter Techniques for Economical, Effective, and Flexible Proteome Analysis
Yi-Han Lin, Rodrigo Vargas Eguez, Isha Vashee, and Yanbao Yu
4. Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry
Ximo Zhang
5. Comprehensive Protocol to Simultaneously Study Protein Phosphorylation, Acetylation, and N-Linked Sialylated Glycosylation
Marcella Nunes Melo-Braga, María Ibáñez-Vea, Katarzyna Kulej, and Martin R. Larsen
6. Phos-Tag Fluorescent Gel Staining for the Quantitative Detection of His- and Asp-Phosphorylated Proteins
Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike
7. Polyubiquitin Profile in Down Syndrome and Alzheimer Disease Brain
Antonella Tramutola and Marzia Perluigi
8. Platform Methods to Characterize the Charge Heterogeneity of Three Common Protein Therapeutics by Imaged Capillary Isoelectric Focusing
Jiaqi Wu, Will McElroy, Charles Haitjema, Carsten Lück, and Christopher Heger
9. A Protocol for Isolation, Purification, Characterization, and Functional Dissection of Exosomes
Alin Rai, Haoyun Fang, Monique Fatmous, Bethany Claridge, Qi Hui Poh, Richard J. Simpson, and David W. Greening
10. Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma
Antonia Reale, Tiffany Khong, Rong Xu, Maoshan Chen, Sridurga Mithraprabhu, Nicholas Bingham, Andrew Spencer, and David W. Greening
11. Isolation of Extracellular Vesicles for Proteomic Profiling
Dongsic Choi, Janusz Rak, and Yong Song Gho
12. Isolation of Proteins from Extracellular Vesicles (EVs) for Mass Spectrometry-Based Proteomic Analyses
Prabal Subedi, Michael Schneider, Michael J. Atkinson, and Soile Tapio
13. Flow Cytometry as an Important Tool in Proteomic Profiling
Michael P. Blundell, Sharon L. Sanderson, and Tracey A. Long
14. Improved Immunoprecipitation to Mass Spectrometry Method for Enrichment of Low-Abundant Protein Targets
Penny Jensen, Bhavin Patel, Suzanne Smith, Renuka Sabnis, and Barbara Kaboord
15. Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors
Jonathan M. Preuss, Ute Burret, and Sabine Vettorazzi
16. Bead-Based Multiplex Immunoassays: Procedures, Tips, and Tricks
Candice Cox
17. Immunoaffinity-Based Liquid Chromatography Mass Spectrometric Assay to Accurately Quantify the Protein Concentration of HMGB1 in EDTA Plasma
Viktoria Anselm, Andreas Steinhilber, Cornelia Sommersdorf, and Oliver Poetz
18. Recombinant Anti-Idiotypic Antibodies in Ligand Binding Assays for Antibody Drug Development
Stefan Harth and Christian Frisch
19. cDNA Display-Mediated Immuno-PCR (cD-IPCR): An Ultrasensitive Immunoassay for Biomolecular Detection
Chathuni Jayathilake and Naoto Nemoto
20. Chromatin Immunoprecipitation (ChIP) to Study DNA-Protein Interactions
Eliza C. Small, Danielle Maryanski, Kevin J. Harvey, Michael-C. Keogh, and Andrea Johnstone
21. Profiling Protein-DNA Interactions by Chromatin Immunoprecipitation in Arabidopsis
Hendry Susila, Zeeshan Nasim, Suhyun Jin, Geummin Youn, Hyewon Jeong, Ji-Yul Jung, and Ji Hoon Ahn
22. Biotin Proximity Labeling for Protein-Protein Interaction Discovery: The BioID Method
Jeff E. Habel
23. Studying OTUD6B-OTUB1 Protein-Protein Interaction by Low-Throughput GFP-Trap Assays and High-Throughput AlphaScreen Assays
Elisabeth Weber, Kenji Schorpp, and Kamyar Hadian
24. Thermal Shift Assay for Exploring Interactions between Fatty Acid-Binding Protein and Inhibitors
Jiaqing Hao
25. Isolation and Purification of Mitochondria from Cell Culture for Proteomic Analyses
Yaschar Kabiri, Christine von Toerne, Adriana Fontes, Percy A. Knolle, and Hans Zischka
26. Investigating the Adipose Tissue Secretome: A Protocol to Generate High Quality Samples Appropriate for Comprehensive Proteomic Profiling
Sonja Hartwig, Jorg Kotzka, and Stefan Lehr
27. Methods for Proteomics-Based Analysis of the Human Muscle Secretome Using an In Vitro Exercise Model
Cora Weigert, Sonja Hartwig, and Stefan Lehr
28. Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Advantages of Stain-Free Technology
Rômulo Leão Silva Neris, Andrea Marie Chua Dobles, and Aldrin V. Gomes
29. Technical Considerations for Contemporary Western Blot Techniques
Kenneth Oh
30. Simple Western: Bringing the Western Blot into the 21st Century
Carsten Lück, Charles Haitjema, and Christopher Heger
31. Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests
R. Ashton Lavoie, Taufika Islam Williams, R. Kevin Blackburn, Ruben G. Carbonell, and Stefano Menegatti
32. Sample Preparation of Secreted Mammalian Host Cell Proteins and their Characterization by Two-Dimensional Electrophoresis and Western Blotting
Anton Posch, Franziska Kollmann, Thomas Berkelman, and Elizabeth Dreskin
33. Quantitative Proteomic Analysis Using Formalin-Fixed Paraffin-Embedded (FFPE) Human Cardiac Tissue
Omid Azimzade1, Mike J. Atkinson, and Soile Tapio
34. Chloroplast Isolation and Enrichment of Low Abundant Proteins by Affinity Chromatography for Identification in Complex Proteomes
Roman G. Bayer, Simon Stael, and Markus Teige
35. Principles of Protein Labeling Techniques
Christian Obermaier, Anja Griebel, and Reiner Westermeier
36. Mechanical/Physical Methods of Cell Disruption and Tissue Homogenization
Stanley Goldberg
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Date de parution : 01-2022
Ouvrage de 592 p.
17.8x25.4 cm
Disponible chez l'éditeur (délai d'approvisionnement : 15 jours).
Prix indicatif 174,06 €
Ajouter au panierDate de parution : 01-2021
Ouvrage de 592 p.
17.8x25.4 cm