Description
Histochemistry of Single Molecules, 1st ed. 2017
Methods and Protocols
Methods in Molecular Biology Series, Vol. 1560
Coordinators: Pellicciari Carlo, Biggiogera Marco
Language: EnglishSubject for Histochemistry of Single Molecules:
Support: Print on demand
Description
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Part I: Vital Histochemistry
1. Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes
Anna L. Peters and Cornelis J.F. van Noorden
2. Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues
Anna C. Croce and Giovanni Bottiroli
3. Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree
Vittorio Bertone, Eleonora Tarantola, and Isabel Freitas
4. Histochemical Demonstration of Tripeptidyl Aminopeptidase I
Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, and Nikolai E. Lazarov
5. Enzyme Histochemistry for Functional Histology in Invertebrates
Francesca Cima
Part II: Lectin Histochemistry
6. Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future
Susan A. Brooks
7. Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads
Ellie-May Beaman, David R.F. Carter, and Susan A. Brooks
8. Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells
Gerrit Wolters-Eisfeld and Udo Schumacher
9. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium
Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Hernández-Martínez, Concepción Ferrer, and Luis Miguel Pastor
Part III: Protein Histochemistry
10. Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications
Shuji Yamashita and Osamu Katsumata
11. Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain
Sara Bonzano and Silvia De Marchis
12. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration
Nicolas A. Dumont and Michael A. Rudnicki
13. Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue
Sabina Berezowska and José A. Galván
Part IV: Lipid Histochemistry
14. Tissue Fixation and Processing for the Histological Identification of Lipids
Víctor Carriel, Fernando Campos, José Aneiros-Fernández, and John A. Kiernan
15. Staining Methods for Normal and Regenerative Myelin in the Nervous System
Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, and Stefano Geuna
16. Nile Red Staining of Neutral Lipids in Yeast
Kerry Ann Rostron and Clare Louise Lawrence
17. Staining of Lipid Droplets with Monodansylpentane
Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, and Wei Yuan Yang
Part V: Nuclear Histochemistry
18. Fluorochromes for DNA Staining and Quantitation
Giuliano Mazzini and Marco Danova
19. Osmium Ammine for Staining DNA at Electron Microscopy
Irene Masiello and Marco Biggiogera
20. DNA Labeling at Electron Microscopy
Nicolas Thelen and Marc Thiry
21. Visualizing RNA at Electron Microscopy by Terbium Citrate
Marco Biggiogera and Irene Masiello
22. Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks
Elva I. Cortés-Gutiérrez, José Luis Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, and Jaime Gosálvez
Part VI: Plant Histochemistry
23. Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins
Malgorzata Ciska and Susana Moreno Díaz de la Espina
24. Histochemical Analysis of Plant Secretory Structures
Diego Demarco
25. A Histochemical Technique for the Detection of Annonaceous Acetogenins
Guillermo Laguna-Hernández, Alicia Enriqueta Brechú-Franco, Iván De la Cruz-Chacón, and Alma Rosa González- Esquinca
Includes cutting-edge techniques for the study of single molecule histochemistry
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
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