Innate DNA and RNA Recognition, 2014 Methods and Protocols Methods in Molecular Biology Series, Vol. 1169

Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources.

Detection of RNA Modifications by HPLC Analysis and Competitive ELISA.- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand.- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis.- Isolation of RIG-I-associated RNAs from Virus Infected Cells.- Structure Modeling of Toll-like Receptors.- Nucleic Acid Recognition in Dendritic Cell.- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems.- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro.- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes.- Mapping of Optimal CD8 T Cell Epitopes.- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection.- MicroRNA Methodology: Advances in miRNA-technologies.- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR).- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus.- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice.- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice.- Bifunctional siRNAs for Tumor Therapy.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts