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This book draws together recent data on both cytoplasmic and flagellar dyneins and the proteins they interact with, to give the reader a clear picture of what is currently known about the structure and mechanics of these remarkable macro-molecular machines. Each chapter is written by active researchers, with a focus on currently used biophysical, biochemical, and cell biological methods. In addition tocomprehensive coverage of structural information gained by electron microscopy, electron cryo-tomography, X-ray crystallography, and nuclear magnetic resonance, this book provides detailed descriptions of mechanistic experiments by single-molecule nanometry.
Dyneins: Ancient Protein Complexes Gradually Reveal Their Secrets. HiroseTwo Decades of Cytoplasmic Dynein: From Fast to Forceful. Functional Analysis of the Dynein Motor Domain. Structural Studies on the Dynein Heavy Chain. Structural Analysis of Microtubule-Bound Dynein. The Dynein Stalk: Atomic Structure and Roles in the Mechanism of the Dynein Motor. Enzymatic and Motile Properties of Native Dynein Molecules. Motile Properties of Cytoplasmic Dynein. Motile Properties of Inner Arm Dynein. Dynein Motility in Cilia and Flagella. 3D Structures of Axonemes. Functional Diversity of Axonemal Dyneins. Assembly and Regulation of Dynein Light Chains. Regulatory Subunits of Axonemal Dynein. The Role of Dynein in Yeast Nuclear Segregation.