Confocal and Two-Photon Microscopy Foundations, Applications and Advances

Confocal and Two-Photon Microscopy
Foundations, Applications, and Advances
Edited by Alberto Diaspro

Confocal and two-photon fluorescence microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits. Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances provides clear, comprehensive coverage of basic foundations, modern applications, and groundbreaking new research developments made in this important area of microscopy.

Opening with a foreword by G. J. Brakenhoff, this reference gathers the work of an international group of renowned experts in chapters that are logically divided into balanced sections covering theory, techniques, applications, and advances, featuring:
* In-depth discussion of applications for biology, medicine, physics, engineering, and chemistry, including industrial applications
* Guidance on new and emerging imaging technology, developmental trends, and fluorescent molecules
* Uniform organization and review-style presentation of chapters, with an introduction, historical overview, methodology, practical tips, applications, future directions, chapter summary, and bibliographical references
* Companion FTP site with full-color photographs
* The significant experience of pioneers, leaders, and emerging scientists in the field of confocal and two-photon excitation microscopy


Confocal and Two-Photon Microscopy: Foundations, Applications, and Advances is invaluable to researchers in the biological sciences, tissue and cellular engineering, biophysics, bioengineering, physics of matter, and medicine, who use these techniques or are involved in developing new commercial instruments.

Foreword (G. Brakenhoff).

Preface.

Acknowledgements.

Contributors.

The Generalized Microscope (C. Sheppard).

Confocal Microscopy: Basic Principles and Architectures (T. Wilson).

Two-Photon Microscopy: Basic Principles and Architectures (A. Diaspro & C. Sheppard).

Cross-Sections of Fluorescent Molecules in Multiphoton Microscopy (C. Xu).

Resolution and Contrast in Confocal and Two-Photon Microscopy (J. Jonkman and E. Stelzer).

The Role of Pinhole Size in High-Aperture Two-and Three-Photon Microscopy (P. Torok & C. Sheppard).

Aberrations and Penetration in In-Depth Confocal and Two-Photon-Excitation Microscopy (C. de Grauw, et al.).

Group Velocity Dispersion and Fiber Delivery in Multiphoton Laser Scanning Microscopy (R. Wolleschensky, et al.).

Cellular and Subcellular Perturbations During Multiphoton Microscopy (K. Konig & U. Tirlapur).

Pracitcal Multiphoton Microscopy (J. Girkin & D. Wokosin).

Sampling, Resolution and Digital Image Processing in the Spatial and Fourier Domains (K. Castleman).

Image-Restoration Methods: Basics and Algorithms (P. Boccacci & M. Bertero).

Two-Photon Excitation Fluorescence Microscopy Imaging in Xenopus and Transgenic Mouse Embryos (A. Periasamy, et al.).

Multiple Color Fluorescence Imaging in Microscopy (K. Castleman).

Stereological Methods for Estimating Geometrical Parameters of Microscopic Structure by Three-Dimensional Imaging (L. Kubinova, et al.).

Imaging Live Cells in 3D Using Wide-Field Microscopy with Image Restoration (W. Carrington).

Confocal Microscopy in the Study of the Cell Nucleus (L. Neri, et al.).

Confocal Imaging of Neuronal Growth and Morphology in Brain Slices (I. Skaliora & S. Pagakis).

FISH Imaging (M. Kozubek).

Two-Photon Imaging of Tissue Physiology based on Endogenous Fluorophores (L. Hsu, et al.).

Two-Photon Near-Infrared Femtosecond Laser Scanning Microscopy in Plant Biology (U. Tirlapur & K. Konig).

Two-Photon Excitation Microscopy for Image Spectroscopy and Biochemistry of Tissues, Cells, Organelles, and Lipid Vesicles Under Physiological Conditions (T. Parasassi, et al.).

Real-Time In Situ Calcium Imaging with Single- and Two-Photon Confocal Microscopy (K. Fujita & T. Takamatsu).

Superresolution in Fluorescence Confocal Microscopy and in DVD Optical Storage (R. Pike).

Photobleaching by Confocal Microscopy (J. McNally & C. Smith).

Two-Photon Laser Scanning Microscopy for Characterization of Integrated Circuits and Optoelectronics (C. Xu).

Index.

Alberto Diaspro was born in Genoa, Italy, on April 7, 1959, and went on to receive his doctoral degree in Electronic Engineering from the University of Genoa in 1983. He is an investigator and professor for the Department of Physics at the University of Genoa, and a member of the National Institute for the Physics of Matter (INFM). Dr. Diaspro also holds positions on the editorial boards of several international journals, namely: Microscopy Research and Technique, Journal of Computer Assisted Microscopy, Journal of Biomedical Optics and European Journal of Histochemistry, and he is the author of more than 100 international scientific publications. His main research activities aim at the study of biostructures both in situ and in vitro, but his many interests include the design, realization and utilization of biophysical instrumentation as conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, and signal and image digital processing.