The Art & Science of Assisted Reproductive Technology

The Art & Science of Assisted Reproductive Technology is a comprehensive step by step guide to different types of ART.

The book is divided into fifteen sections, beginning with instructions on setting up an assisted reproductive technology laboratory. The second section is a guide to the evaluation of an infertile couple, including the use of ultrasound and Doppler imaging in infertility, and the effect of the environment on fertility. Further sections include information on third party reproduction, medical and surgical treatments, and gamete donation and manipulation.

The book describes ART techniques that employ molecular genetics, which can maximise the efficiency of ART treatments. Conditions that negatively affect fertility, including sexually transmitted diseases, endometriosis, ectopic pregnancy and immune system conditions are also discussed in detail.

The Art & Science of Assisted Reproductive Technology covers all aspects of ART, from laboratory set up, embryo transfer and implantation, to cryopreservation (freezing) of eggs, sperm and embryos. Enhanced by nearly 300 full colour images, illustrations and tables, this book an essential resource for gynaecologists and infertility specialists.

Key Points

• Comprehensive step by step guide to all aspects of assisted reproductive technology
• Includes guide to set up of an ART laboratory
• Information on ART in special conditions, third party ART and complications in ART
• 287 full colour images, illustrations and tables

Section 1 Setting-up an ART Laboratory

• How to Set-up an ART Laboratory
• Quality Control in an ART Laboratory
• Quality Care Management in ART Clinic

Section 2 How to Investigate an Infertile Couple

• Evaluation of the Male Partner
• Investigation of Infertile Female
• Ultrasound and Doppler in Infertility
• Effect of Environment on Fertility

Section 3 Endoscopy in Infertility

• Fertility Enhancing Laparoscopic Surgery
• Fertility Enhancing Hysteroscopic Surgery
• Pilot Hysteroscopy before ART

Section 4 Endocrinology and Stimulation Protocols

• Genetic Markers of Ovarian Response
• Physiology of Follicular Phase
• Progesterone in Follicular Phase during Controlled Ovarian Stimulation
• Therapeutic Window for Luteinizing Hormone in Controlled Ovarian Stimulation
• Stimulation Protocols for ART
• Stimulation Protocols for Polycystic Ovarian Syndrome in ART
• GnRH Agonists and Antagonists in Assisted Reproduction
• Monitoring Ovarian Stimulation
• Ovulation Trigger

Section 5 Gamete Collection and Preparation

• Oocyte Retrieval
• Oocyte Evaluation and Preparation
• Semen Analysis and Evaluation of Sperm
• Semen Collection and Preparation

Section 6 Micromanipulation

• Micromanipulation: Instrument, Technique and ICSI Procedure

Section 7 Enhancing Sperm, Oocyte, Embryo Quality

• Polarized Light Microscopy
• IMSI in Daily Laboratory Practice: Lights and Shadows
• Role of Preimplantation Genetic Diagnosis in ART
• Assisted Hatching of Human Embryos

Section 8 Culture of Embryos and Transfer

• Culture and Evaluation of Embryos: From Fertilization to Transfer
• Proteomics and Metabolomics in ART
• Time-lapse Microscopy
• Embryo Transfer: Catheters and Techniques

Section 9 Implantation

• Endometrial Receptivity
• Embryo Endometrial Dialogue
• Physiology of Implantation in Natural Cycle versus Stimulated Cycle
• Recurrent Implantation Failure: Strategies and Intervention
• Implantation and the Endometrium in Uterine Fibroid
• Implantation in Endometriosis

Section 10 Cryopreservation

• Oocyte Cryopreservation
• Embryo Cryopreservation
• Cryopreservation of Ovarian Tissue
• In Vitro Maturation of Oocytes
• Semen Banking
• Fertility Preservation in Cancer (Male and Female)

Section 11 Luteal Phase in ART

• Luteal Phase Support in ART

Section 12 ART in Special Conditions

• Endometriosis and ART
• Myomas in Infertility
• Genital Tuberculosis and ART
• Poor Ovarian Response
• ART in Polycystic Ovary Syndrome

Section 13 Third Party ART

• Gamete Donation
• Embryo Donation
• Surrogacy

Section 14 Pregnancy and ART

• First Trimester Screening in Pregnancies Conceived with ART
• Obstetric Outcome Following ART
• ART to Antenatal Care

Section 15 Complications in ART

• Ovarian Hyperstimulation Syndrome
• Ovarian Cancer and ART
• Ethical and Stress Issues in ART
• Index

Sunita R Tandulwadkar MD FICS FICOG
Chief, Ruby Hall IVF and Endoscopy Centre, Head, Department of Obstetrics and Gynaecology, Ruby Hall Clinic, Pune, Maharashtra, India