Biobanking, 1st ed. 2019 Methods and Protocols Methods in Molecular Biology Series, Vol. 1897

This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Unique and comprehensive, Biobanking: Methods and Protocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.

Preface…

Table of Contents…

Contributing Authors… 

1. Sustainability in Biobanking  

Maram Abdaljaleel, Elyse J. Singer, and William H. Yong 

 

2. An Introduction to Starting a Biobank 

Mitra D. Harati, Ryan R. Williams, Masoud Movassaghi, Amin Hojat, Greg Lucey, William H. Yong 

 

3. An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks  

Kyuseok Im, Dorina Gui, and William H. Yong

4. Disaster Prevention and Recovery 

Chon Boon Eng and Weiling Tan

5. Minority Participation in Biobanks: An Essential Key to Progress

Paula Kim and Erin L. Milliken

6. Orientation and Training of New Biobank Personnel  

Ryan R. Williams, Diviya Gupta, and William H. Yong

7. Procurement and Storage of Surgical Biospecimens 

Amin Hojat, Bowen Wei, Madeline G. Olson, Qinwen Mao, and William H. Yong

  

8. Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization  

Randy S. Tashjian, Ryan R. Williams, Harry V. Vinters, and William H. Yong

9. Procurement, Storage, and Use of Blood in Biobanks 

Jaclyn N. Perry, Afreen Jasim, Amin Hojat, and William H. Yong

10. Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens 

Jennifer Woo and David Y. Lu

11. Biobanking of Cerebrospinal Fluid 

Randy S. Tashjian, Harry V. Vinters, and William H. Yong

12. Biobanking of Urine Samples

Neda A. Moatamed

13. Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking 

Alberto M. Marchevsky, Shikha Bose, and Beatrice Knudsen

14. The Fluid Preservation of Zoological Specimens

Thomas P.V. Hartman

15. Photographing Fluid Preserved Specimens 

David Glynne Fox and Thomas P.V. Hartman

16. Casts of Fluid Preserved Specimens

Sarah Burhouse and Thomas P.V. Hartman

17. Collection and Preservation of Terrestrial Arthropods 

Jeffrey D. Whitman, Douglas Yanega, Cole B.G. Watson, and Vincent W. Strode

18. Procurement and Preservation of Plants 

Allison D. Rudalevige and Jeffrey D. Whitman

19. Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank 

Lisa Ta, Laura Gosa, and David A. Nathanson

20. Decontamination of Biobank Facilities 

Xinhai Zhang, Saied Mirshahidi, and Chien-Shing Chen

21. Laboratory Safety: Chemical and Physical Hazards 

Saeed Asiry and Lee-Cyn Ang

22. Making Formalin-Fixed Paraffin Embedded Blocks 

Alireza Sadeghipour and Pegah Babaheidarian

  

23. Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections 

Joanne Sy and Lee Cyn Ang

24. Performing and Cutting Frozen Sections

25. An Introduction to the Performance of Immunohistochemistry

Shino Magaki, Seyed A. Hojat, Bowen Wei, Alexandra So, and William H. Yong

 

26. An Introduction to Performing Immunofluorescence Staining

Kyuseok Im, Sergey Mareninov, M. Fernando Palma Diaz, and William H. Yong

27. Making a Tissue Microarray

Matthew Koo, Jill M. Squires, Daphne Ying, and Jiaoti Huang

28. Nucleic Acid Isolation and Quality Control

Ling Dong, Janice Yoshizawa, and Xinmin Li

29. Fundamentals of RNA Analysis on Biobanked Specimens

Samuel P. Strom

  

30. Nucleic Acid Extraction from Human Biological Samples

Sureni V. Mullegama, Michael O. Alberti, Cora Au, Yan Li, Traci Toy, Vanina Tomasian, and Rena R. Xian

31. An Overview of DNA Analytical Methods

Valerie A. Arboleda and Rena R. Xian

32. Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs)

Pete Heinzelman, David N. Powers, James A. Wohlschlegel, and Varghese John

33. Sample Preparation for Transmission Electron Microscopy

Parastou Tizro, Cecilia Choi, and Negar Khanlou

34. Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines   

Laura Gosa, Lisa Ta, and David A. Nathanson 

 

35. Domestic and International Shipping of Biospecimens

Dominique Gordy, Randy S. Tashjian, Hannah Lee, Masoud Movassaghi, and William H. Yong