Experimental and applied physiology lab manual (7th ed )

A comprehensive, stand-alone laboratory manual for the one-semester physiology course taught at the undergraduate level. It can accompany any physiology textbook on the market. It reinforces those principles that are fundamental to all courses on physiology. The strengths of this lab manual are its emphasis of hands-on experiments, a practical balance of background information, and clear procedural instructions.

1 Metrics, Measurements, Computations 2 Bioinstrumentation 3 Compound Light Microscopy 4 Physical Processes of Biological Importance 5 Neural Control of Skeletal Muscle 6 Contractility of Skeletal Muscle I: The Twitch and Motor Unit Summation 7 Contractility of Skeletal Muscle II: Mechanical Summation, Contracture, Tetanus, and Fatigue 8 Contractility of Skeletal Muscle III: Isotonic Contraction and Initial Length versus Work 9 Electromyography and Dynamometry 10 Neuromuscular Reflexes of the Spinal Cord and Brain Stem 11 Somatic Sensation 12 Cranial Nerves: Assessment of Functions 13 Visual Acuity, Accommodation, Peripheral Vision, Color Vision, and Ophthalmoscopy 14 Hearing and Equilibrium 15 Electroencephalography I: Relaxation and Brain Rhythms 16 Electroencephalography II: Occipital Lobe Alpha Rhythms 17 Blood Cells and Blood Types 18 Red Blood Cell Count 19 Hemoglobin Content, Hematocrit, and Red Cell Indices 20 Hemostasis: The Platelet Count, Bleeding Time, and Coagulation 21 White Blood Cell Count 22 Microcirculation 23 The Cardiac Cycle 24 Heart Sounds, Pulse Rate, and Systemic Blood Pressure 25 Plethysmography and the Peripheral Pressure Pulse 26 Electrical Activity of the Heart: The Electrocardiogram 27 Electrical Activity of the Heart: Vectorcardiography 28 The Respiratory Cycle 29 Pulmonary Function Tests: Volumes and Capacities 30 Pulmonary Function Tests: Forced Expiratory Volume and Maximal Voluntary Ventilation 31 Analysis of Urine 32 Digestion 33 Metabolic Rates: Indirect Calorimetry 34 Maintenance and Regulation of Body Temperature 35 Glucose Tolerance Appendix A Preparation of Agar Plates and Pipettes Appendix B Preparation of the Egg Osmometer Appendix C Pithing the Frog Appendix D Preparing the Freshwater Turtle for Heart Experiments Appendix E Jones Pulmonor Disinfectant Procedure